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Ca Ski
Ca Ski
规格:
货期:
编号:TS200225
品牌:Testobio
产品名称： Ca Ski
商品货号： TS200225
Organism： Homo sapiens, human
Tissue： cervix; Derived fom metastatic site: small intestine
Cell Type： epithelial cell (CRM)
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 2
Cells contain human papilloma virus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： epidermoid carcinoma
Age： 40 years adult
Gender： female
Ethnicity： Caucasian
Applications：
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.xa0
Intended Use：

Certified Reference Material produced under an ISO 17034 accredited process.


xa0xa0xa0 ACLASS Accredited Reference Material Producer AR-1384
Storage Conditions： liquid nitrogen vapor phase
Derivation：
The line was established from cells from a metastasis in the small bowel mesentery.
Clinical Data：
female
Caucasian
40 years adult
Genes Expressed：
beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen
Cellular Products：
beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen
Comments：

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

The cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18.

NOTE: Virology taxonomy has discontinued use of the family name "Papovaviridae," which includes the term "Papovavirus." Instead, this former family name, "Papovaviridae," has now been split into two distinct and separate families, "Papillomaviridae" and "Polyomaviridae." As such, all cultures containing HPV should be noted to contain "Papillomavirus" (signifying its origin from the "Papillomaviridae" family) instead of the former "Papovavirus."
Complete Growth Medium： The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation：
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37°C
STR Profile：
Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17
Isoenzymes：
G6PD, B
Name of Depositor： RA Pattillo
Deposited As： Homo sapiens
References：

Pattillo RA, et al. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196: 1456-1458, 1977. PubMed: 867042

Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

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Ca Ski

  • 货号: TS200225
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Ca Ski
商品货号： TS200225
Organism： Homo sapiens, human
Tissue： cervix; Derived fom metastatic site: small intestine
Cell Type： epithelial cell (CRM)
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 2
Cells contain human papilloma virus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： epidermoid carcinoma
Age： 40 years adult
Gender： female
Ethnicity： Caucasian
Applications：
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.xa0
Intended Use：

Certified Reference Material produced under an ISO 17034 accredited process.


xa0xa0xa0 ACLASS Accredited Reference Material Producer AR-1384
Storage Conditions： liquid nitrogen vapor phase
Derivation：
The line was established from cells from a metastasis in the small bowel mesentery.
Clinical Data：
female
Caucasian
40 years adult
Genes Expressed：
beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen
Cellular Products：
beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen
Comments：

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

The cells are reported to contain an integrated human papillomavirus type 16 genome (HPV-16, about 600 copies per cell) as well as sequences related to HPV-18.

NOTE: Virology taxonomy has discontinued use of the family name "Papovaviridae," which includes the term "Papovavirus." Instead, this former family name, "Papovaviridae," has now been split into two distinct and separate families, "Papillomaviridae" and "Polyomaviridae." As such, all cultures containing HPV should be noted to contain "Papillomavirus" (signifying its origin from the "Papillomaviridae" family) instead of the former "Papovavirus."
Complete Growth Medium： The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation：
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37°C
STR Profile：
Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17
Isoenzymes：
G6PD, B
Name of Depositor： RA Pattillo
Deposited As： Homo sapiens
References：

Pattillo RA, et al. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196: 1456-1458, 1977. PubMed: 867042

Baker CC, et al. Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. J. Virol. 61: 962-971, 1987. PubMed: 3029430

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

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