pRY255
pRY255
规格:
货期:
编号:TS200254
品牌:Testobio
| 产品名称： | pRY255 |
|---|---|
| 商品货号： | TS200254 |
| Designations： | pRY255 |
| Depositors： | RR Yocum |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli YMC9 |
| Vector Information： | Size (kb): 17.8999996185302700 Vector: pRY255 (plasmid) Promoters: Promoter CYC1 Construction: pRY253 Marker(s):ampR G418R Construct size (kb): 17.89999961853027 Features: marker(s): G418R marker(s): ampR other: HO promoter: CYC1 replicon: pMB1 coding sequence: GAL1/lacZ fusion |
| Applications： | YI-type (integrating) shuttle vector vector permitting positive selection for integration vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--11.0, 7.4; KpnI--19.0; SalI--19.0. A YI-type (yeast integrating, HO gene) shuttle vector that confers G418 resistance under control of the CYC1 promoter. Expresses a GAL1/lacZ fusion product allowing color selection. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pRY255 |
|---|---|
| 商品货号： | TS200254 |
| Designations： | pRY255 |
| Depositors： | RR Yocum |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli YMC9 |
| Vector Information： | Size (kb): 17.8999996185302700 Vector: pRY255 (plasmid) Promoters: Promoter CYC1 Construction: pRY253 Marker(s):ampR G418R Construct size (kb): 17.89999961853027 Features: marker(s): G418R marker(s): ampR other: HO promoter: CYC1 replicon: pMB1 coding sequence: GAL1/lacZ fusion |
| Applications： | YI-type (integrating) shuttle vector vector permitting positive selection for integration vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--11.0, 7.4; KpnI--19.0; SalI--19.0. A YI-type (yeast integrating, HO gene) shuttle vector that confers G418 resistance under control of the CYC1 promoter. Expresses a GAL1/lacZ fusion product allowing color selection. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
