| 产品名称： | pBV730ts |
|---|---|
| 商品货号： | TS200519 |
| Designations： | pBV730ts |
| Depositors： | Wisconsin Alumni Res. Fndn. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 16.2000007629394500 Vector: pBV730ts (plasmid) Construction: pMT660, S. achromogenes amplifiable unit of DNA (AUD-Sar 1) Marker(s):spcR,tsrR Construct size (kb): 16.20000076293945 Features: marker(s): spcR, tsrR |
| Applications： | contains sequence DNA Segment (amplifiable unit of DNA, AUD) vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--16.2; SphI--8.0, 6.2, 1.9; BamHI--16.2. The S. achromogenes fragment contains the following restriction sites (approximate kb from one end): MluI--1.7, 2.5, 3.0, 5.4, 5.9, 9.4, 10.2; SphI--2.1, 9.7; BglII--8.0. When grown at 28C in Streptomyces lividans, the plasmid is present at high copy number and permits expression of spectinomycin resistance without amplification. When grown at 39C, the plasmid is cured and streptomycin resistance is lost, except when AUD-Sar 1 sequences become integrated into the host chromosome where they undergo amplification. Curable vector that can be used to insert and amplify cloned sequences into host chromosomal DNA. The 10.4 kb fragment from S. achromogenes contains the complete 8.8 kb amplifiable unit with 0.8 kb direct repeats at its termini. The unique BglII site is at one end of the 1.7 kb SphI/BglII fragment that determines spectinomycin resistance. pMT660 is a temperature sensitive replication mutant of pIJ702. Distributed in aliquots of 200 ng dried. |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Hornemann U, et al. Spectinomycin resistance and associated DNA amplification in Streptomyces achromogenes subsp. rubradiris. J. Bacteriol. 169: 2360-2366, 1987. PubMed: 3034854 Hornemann U, et al. Recombinant vector having a Streptomcyes achromogenes DNA sequence useful for gene amplification. US Patent 5,240,858 dated Aug 31 1993 Hornemann U, et al. Transferable Streptomyces DNA amplification and coamplification of foreign DNA sequences. J. Bacteriol. 175: 1126-1133, 1993. PubMed: 8432707 |
| Shipped： | freeze-dried |
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| 产品名称： | pBV730ts |
|---|---|
| 商品货号： | TS200519 |
| Designations： | pBV730ts |
| Depositors： | Wisconsin Alumni Res. Fndn. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 16.2000007629394500 Vector: pBV730ts (plasmid) Construction: pMT660, S. achromogenes amplifiable unit of DNA (AUD-Sar 1) Marker(s):spcR,tsrR Construct size (kb): 16.20000076293945 Features: marker(s): spcR, tsrR |
| Applications： | contains sequence DNA Segment (amplifiable unit of DNA, AUD) vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--16.2; SphI--8.0, 6.2, 1.9; BamHI--16.2. The S. achromogenes fragment contains the following restriction sites (approximate kb from one end): MluI--1.7, 2.5, 3.0, 5.4, 5.9, 9.4, 10.2; SphI--2.1, 9.7; BglII--8.0. When grown at 28C in Streptomyces lividans, the plasmid is present at high copy number and permits expression of spectinomycin resistance without amplification. When grown at 39C, the plasmid is cured and streptomycin resistance is lost, except when AUD-Sar 1 sequences become integrated into the host chromosome where they undergo amplification. Curable vector that can be used to insert and amplify cloned sequences into host chromosomal DNA. The 10.4 kb fragment from S. achromogenes contains the complete 8.8 kb amplifiable unit with 0.8 kb direct repeats at its termini. The unique BglII site is at one end of the 1.7 kb SphI/BglII fragment that determines spectinomycin resistance. pMT660 is a temperature sensitive replication mutant of pIJ702. Distributed in aliquots of 200 ng dried. |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Hornemann U, et al. Spectinomycin resistance and associated DNA amplification in Streptomyces achromogenes subsp. rubradiris. J. Bacteriol. 169: 2360-2366, 1987. PubMed: 3034854 Hornemann U, et al. Recombinant vector having a Streptomcyes achromogenes DNA sequence useful for gene amplification. US Patent 5,240,858 dated Aug 31 1993 Hornemann U, et al. Transferable Streptomyces DNA amplification and coamplification of foreign DNA sequences. J. Bacteriol. 175: 1126-1133, 1993. PubMed: 8432707 |
| Shipped： | freeze-dried |
