| 产品名称： | pHGN3 |
|---|---|
| 商品货号： | TS200665 |
| Designations： | pHGN3 |
| GenBank Number： | M14783 |
| Species： | Vaccinia virus |
| Depositors： | University of Florida, Gainesville, RW Moyer, University of Florida, Gainesville |
| Applications： | contains sequence hemagglutinin integrating vector shuttle vector vector permitting positive selection for integration vector with sequences for protein export |
| Vector： | Name of vector: pUC9 Features (with orientation and position when available): Cross references: DNA Seq. Acc.: L09128 |
| Insert： | Genome: Vaccinia virus Gene name: hemagglutinin Contains complete coding sequence?: U Strain: IHD-J (ATCC VR-156) Type of DNA: genomic Insert end:xa0xa0 Modification: KpnI linkers Insert end:xa0xa0 Modification: KpnI linkers Insert size (kb): 1.85 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI--4.2; SalI/HindIII--3.2, 1.0. Recombinants can be identified by inactivation of the hemagglutinin gene (HA-). Shuttle vector component of a virulence tester system to be used with the avirulent rabbit poxvirus deletion mutant RPmuhr23HA+ (ATCC VR-2379). DNA cloned into the MCS is integrated into VR-2379 by homologous recombination, possibly altering the host range and virulence of the virus. The hemagglutinin insert is a 1.8 kb 5SalI/3HindIII fragment containing approximately 443 bp 5 flanking sequence, 988 bp coding sequence interrupted by a polylinker, and 408 bp 3 flanking sequence. The gene is interrupted at the AccI site (nt 43 of the coding region) by a 47 bp MCS from pUC9. Constructed by deleting lacZ and the polylinker from pUC9 by a HaeII digest. The order of the major features in the plasmid is: pUC9 delta lacZ - KpnI - 5 end of insert - PstI/MCS/EcoRI - 3 end of insert - KpnI. |
| Classification： | Poxviridae, Orthopoxvirus |
| References： | Moyer RW, Bloom DC. Biological system for constructing and testing viral vaccines. US Patent 5,212,057 dated May 18 1993 Bloom DC, et al. Identification and characterization of two nonessential regions of the rabbit pox virus genome involved in virulence. J. Virol. 65: 1530-1542, 1991. PubMed: 1847470 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pHGN3 |
|---|---|
| 商品货号： | TS200665 |
| Designations： | pHGN3 |
| GenBank Number： | M14783 |
| Species： | Vaccinia virus |
| Depositors： | University of Florida, Gainesville, RW Moyer, University of Florida, Gainesville |
| Applications： | contains sequence hemagglutinin integrating vector shuttle vector vector permitting positive selection for integration vector with sequences for protein export |
| Vector： | Name of vector: pUC9 Features (with orientation and position when available): Cross references: DNA Seq. Acc.: L09128 |
| Insert： | Genome: Vaccinia virus Gene name: hemagglutinin Contains complete coding sequence?: U Strain: IHD-J (ATCC VR-156) Type of DNA: genomic Insert end:xa0xa0 Modification: KpnI linkers Insert end:xa0xa0 Modification: KpnI linkers Insert size (kb): 1.85 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI--4.2; SalI/HindIII--3.2, 1.0. Recombinants can be identified by inactivation of the hemagglutinin gene (HA-). Shuttle vector component of a virulence tester system to be used with the avirulent rabbit poxvirus deletion mutant RPmuhr23HA+ (ATCC VR-2379). DNA cloned into the MCS is integrated into VR-2379 by homologous recombination, possibly altering the host range and virulence of the virus. The hemagglutinin insert is a 1.8 kb 5SalI/3HindIII fragment containing approximately 443 bp 5 flanking sequence, 988 bp coding sequence interrupted by a polylinker, and 408 bp 3 flanking sequence. The gene is interrupted at the AccI site (nt 43 of the coding region) by a 47 bp MCS from pUC9. Constructed by deleting lacZ and the polylinker from pUC9 by a HaeII digest. The order of the major features in the plasmid is: pUC9 delta lacZ - KpnI - 5 end of insert - PstI/MCS/EcoRI - 3 end of insert - KpnI. |
| Classification： | Poxviridae, Orthopoxvirus |
| References： | Moyer RW, Bloom DC. Biological system for constructing and testing viral vaccines. US Patent 5,212,057 dated May 18 1993 Bloom DC, et al. Identification and characterization of two nonessential regions of the rabbit pox virus genome involved in virulence. J. Virol. 65: 1530-1542, 1991. PubMed: 1847470 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
