| 产品名称： | 70-38 |
|---|---|
| 商品货号： | TS201095 |
| Designations： | 70-38 |
| Species： | Mus musculus, mouse |
| Depositors： | CM Disteche |
| Applications： | Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct. |
| Vector： | Construct size (kb): 14.60000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 9.0 Gene product: DNA segment, Chr X, University of Washington-70 DXWas70 |
| Insert Size (kb)： | 9.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 5.8; BamHI--6.4, 5.6, 2.25, 0.7; PstI--14.8; BglI--9.4, 2.4, 1.1 (doublet), 0.8; HindIII--11.0, 2.0, 1.4, 0.4. In the strains that demonstrate amplification, variant bands are detected in Southern blots when genomic DNA is digested with MspI, HindIII, BglII, TaqI, BglI, BclI, MboI and EcoRI. This sequence exists in about 20 copies in strains A/J, AKR/J, BALB/cJ, CBA/J, C3H/HeJ, DBA/2J, SWR/J, and C57BL/6J (all on the X chromosome). In other strains, the number of copies is 80 - 100, with some of the additional sequences being on chromsome 11 (C57BL/10ScSn mdx, C57BL/10J), chromosome 17 (SJL/J, PO) or chromosome 13 (NZB/B1NJ). Hybridizes to the X chromosome of Mus caroli. Also detects multiple bands in the human genome. Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Disteche CM, et al. Comparison of the physical and recombination maps of the mouse X chromosome. Genomics 5: 177-184, 1989. PubMed: 2571569 Disteche CM, et al. Isolation of mouse X-chromosome specific DNA from an X-enriched lambda phage library derived from flow sorted chromosomes. Cytometry 2: 282-286, 1982. PubMed: 6210515 Disteche CM, et al. Isolation and characterization of two repetitive DNA fragments located near the centromere of the mouse X chromosome. Cytogenet. Cell Genet. 39: 262-268, 1985. PubMed: 2932308 Disteche CM, et al. Translocation and amplification of an X-choromosome DNA repeat in inbred strains of mice. Nucleic Acids Res. 15: 4393-4401, 1987. PubMed: 3588301 |
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| 产品名称： | 70-38 |
|---|---|
| 商品货号： | TS201095 |
| Designations： | 70-38 |
| Species： | Mus musculus, mouse |
| Depositors： | CM Disteche |
| Applications： | Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct. |
| Vector： | Construct size (kb): 14.60000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 9.0 Gene product: DNA segment, Chr X, University of Washington-70 DXWas70 |
| Insert Size (kb)： | 9.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 5.8; BamHI--6.4, 5.6, 2.25, 0.7; PstI--14.8; BglI--9.4, 2.4, 1.1 (doublet), 0.8; HindIII--11.0, 2.0, 1.4, 0.4. In the strains that demonstrate amplification, variant bands are detected in Southern blots when genomic DNA is digested with MspI, HindIII, BglII, TaqI, BglI, BclI, MboI and EcoRI. This sequence exists in about 20 copies in strains A/J, AKR/J, BALB/cJ, CBA/J, C3H/HeJ, DBA/2J, SWR/J, and C57BL/6J (all on the X chromosome). In other strains, the number of copies is 80 - 100, with some of the additional sequences being on chromsome 11 (C57BL/10ScSn mdx, C57BL/10J), chromosome 17 (SJL/J, PO) or chromosome 13 (NZB/B1NJ). Hybridizes to the X chromosome of Mus caroli. Also detects multiple bands in the human genome. Isolated from a flow-sorted library enriched for the derivative X(7) chromosome produced by the Cattanach translocation T(X;7)1Ct. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Disteche CM, et al. Comparison of the physical and recombination maps of the mouse X chromosome. Genomics 5: 177-184, 1989. PubMed: 2571569 Disteche CM, et al. Isolation of mouse X-chromosome specific DNA from an X-enriched lambda phage library derived from flow sorted chromosomes. Cytometry 2: 282-286, 1982. PubMed: 6210515 Disteche CM, et al. Isolation and characterization of two repetitive DNA fragments located near the centromere of the mouse X chromosome. Cytogenet. Cell Genet. 39: 262-268, 1985. PubMed: 2932308 Disteche CM, et al. Translocation and amplification of an X-choromosome DNA repeat in inbred strains of mice. Nucleic Acids Res. 15: 4393-4401, 1987. PubMed: 3588301 |
