| 产品名称： | pLOI292 |
|---|---|
| 商品货号： | TS201434 |
| Designations： | pLOI292 |
| Species： | Zymomonas mobilis (Lindner) Kluyver and van Niel |
| Depositors： | University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville |
| Applications： | produces protein alcohol dehydrogenase II produces protein pyruvate decarboxylase |
| Vector： | Construct size (kb): 16.36000061035156 |
| Insert： | DNA: genomic Insert lengths(kb): 3.099999904632568 Gene product: alcohol dehydrogenase II pdc Target Gene: pyruvate decarboxylase, alcohol dehydrogenase II |
| Insert Size (kb)： | 3.100 |
| Media： | 1675 plus glucose (2%)
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--13.4, 3.1; EcoRI--16.5; HindIII--9.8, 3.9, 1.6, 1.0. The GenBank accession number for the adh insert is M15394. The vector contains oriV from RSF1010, mob sequences from RSF1010 and pSUP205, and a Zymomonas mobilis promoter upstream of the BamHI cloning site. Constructed by cloning a blunted EcoRI/SalI fragment containing both pdc and adhB into the SmaI site of pUC19. The BamHI fragment from this construct was cloned into the vector, such that both genes are controlled by the Z. mobilis promoter. |
| References： | Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991 Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063 Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037 Conway T, et al. Expression vector for Zymomonas mobilis. Appl. Environ. Microbiol. 53: 235-241, 1987. Ingram LO, Conway T. Expression of different levels of ethanologenic enzymes from Zymomonas mobilis in recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 54: 397-404, 1988. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pLOI292 |
|---|---|
| 商品货号： | TS201434 |
| Designations： | pLOI292 |
| Species： | Zymomonas mobilis (Lindner) Kluyver and van Niel |
| Depositors： | University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville |
| Applications： | produces protein alcohol dehydrogenase II produces protein pyruvate decarboxylase |
| Vector： | Construct size (kb): 16.36000061035156 |
| Insert： | DNA: genomic Insert lengths(kb): 3.099999904632568 Gene product: alcohol dehydrogenase II pdc Target Gene: pyruvate decarboxylase, alcohol dehydrogenase II |
| Insert Size (kb)： | 3.100 |
| Media： | 1675 plus glucose (2%)
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--13.4, 3.1; EcoRI--16.5; HindIII--9.8, 3.9, 1.6, 1.0. The GenBank accession number for the adh insert is M15394. The vector contains oriV from RSF1010, mob sequences from RSF1010 and pSUP205, and a Zymomonas mobilis promoter upstream of the BamHI cloning site. Constructed by cloning a blunted EcoRI/SalI fragment containing both pdc and adhB into the SmaI site of pUC19. The BamHI fragment from this construct was cloned into the vector, such that both genes are controlled by the Z. mobilis promoter. |
| References： | Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991 Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063 Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037 Conway T, et al. Expression vector for Zymomonas mobilis. Appl. Environ. Microbiol. 53: 235-241, 1987. Ingram LO, Conway T. Expression of different levels of ethanologenic enzymes from Zymomonas mobilis in recombinant strains of Escherichia coli. Appl. Environ. Microbiol. 54: 397-404, 1988. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
