Fibroblast Growth Kit-Low serum

规格:

货期:

编号:TS201556

品牌:Testobio

产品名称：

Fibroblast Growth Kit-Low serum

商品货号：

TS201556

Biosafety Level：

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications：

The kit contains components that when added to the fibroblst basal medium ATCC PCS-201-030 create a complete culture environment for fibroblasts. Use of this kit will support a faster rate of proliferation compared to when Fibroblast Growth Kit, Serum-free (ATCC PCS-201-040) is used.

Product Format：

frozen 1 Kit

Storage Conditions：

-20°C (or -70°C for long-term storage)

Comments：

An upper limit for population doublings has not been established for primary fibroblasts when cultured with Fibroblast Basal Medium supplemented with Fibroblast Growth Kit, Low serum (2% FBS).
The final concentration for each component is as follows:

L-glutamine: 7.5 mM
rh FGF basic: 5 ng/mL
rh Insulin: 5 µg/mL
Hydrocortisone: 1 µg/mL
Ascorbic acid: 50 µg/mL
Fetal bovine serum: 2%

Complete Growth Medium：

Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates, prior to adding to the basal medium.
Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC^® PCS-201-040), add the indicated volume for each component in the order shown.

Component
Volume：	Volume
Sterility Tests：	Final Concentration
Functional Tests：	L-glutamine
C of A：	18.75 mL

序号	文章标题	操作
1	ATCC细胞运输和处理	查看详情
2	其他海外品牌代理	查看详情
3	ATCC代理	查看详情
4	购买须知	查看详情
5	订购流程	查看详情

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Fibroblast Growth Kit-Low serum

货号: TS201556
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品牌 : TESTOBIO

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产品名称：

Fibroblast Growth Kit-Low serum

商品货号：

TS201556

Biosafety Level：

Applications：

Product Format：

frozen 1 Kit

Storage Conditions：

-20°C (or -70°C for long-term storage)

Comments：

L-glutamine: 7.5 mM
rh FGF basic: 5 ng/mL
rh Insulin: 5 µg/mL
Hydrocortisone: 1 µg/mL
Ascorbic acid: 50 µg/mL
Fetal bovine serum: 2%

Complete Growth Medium：

Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates, prior to adding to the basal medium.
Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC^® PCS-201-040), add the indicated volume for each component in the order shown.