| 产品名称： | Botryotinia fuckeliana (de Bary) Whetzel, teleomorph |
|---|---|
| 商品货号： | TS201589 |
| Deposited As： | Botryotinia fuckeliana (de Bary) Whetzel |
| Strain Designations： | SAS405 |
| Alternate State： | Botrytis cinerea Persoon, anamorph |
| Application： | Produces glucan 1,3-beta-glucosidase beta-D-1,3-glucanase, exo-1,3-beta-glucanase, exo-1,3-beta-glucosidase Produces extracellular 1,3-beta-glucanases |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | MAT1-2 Mbc1HR Daf1LR |
| Preceptrol®： | no |
| Ploidy： | haploid |
| Comments： | Mating system
Growth in culture
Genetics of fungicide resistance |
| Morphology： | Colonies at 25°C on Potato sucrose agar after 4-5 days are greyish brown and sclerotia black small. Conidia ellipsoidal and smooth. Place culture under UV light for sporulation. |
| Medium： | ATCC® Medium 324: Malt extract agar ATCC® Medium 338: Potato sucrose agar |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGGGTAGACCTCCCACCCTTGTGTATTATTACTTTGTTGCTTTGGCGAGCTGCCTTCGGGCCTTGTATGCTCGCCAGAGAATACCAAAACTCTTTTTATTAATGTCGTCTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTTAGCTTGGTATTGAGTCTATGTCAGTAATGGCAGGCTCTAAAATCAGTGGCGGCGCCGCTGGGTCCTGAACGTAGTAATATCTCTCGTTACAGGTTCTCGGTGTGCTTCTGCCAAAACCCAAATTTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTACCTCAGTAACGGCGAGTGAAGCGGTAAAAGCTCAAATTTGAAATCTGGCTCTTTTAGAGTCCGAATTGTAATTTGTAGAAGATGCTTCGGGTGTGGTTCCGGTCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGTGACTGGATACCTATGCTCATGTGAAGCTCTTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGAGGTATATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGCAATCAGACTTGCACTTGGTGTTCATCAGGGTCTCGTACCCTGTGTACTTCATCAAGTTCAGGCCAGCATCAGTTTGAGTGGTTAGATAAAGGCTTAGAGAATGTGGCCCTCTTCGGGGGGTGTTATAGCTCTAGGTGCAATGTAGCCTACTTGGACTGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGAC |
| Name of Depositor： | F Faretra |
| Isolation： | Single-ascospore isolate |
| Cross References： | Nucleotide (GenBank) : KP780449 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KP780471 D1/D2 region of 28S rRNA |
| References： | Staats M, et al. Positive selection in phytotoxic protein-encoding genes of Botrytis species. Fungal Genet Biol 44: 52-63, 2007. PubMed: 16935013 Staats M, van Baarlen P, van Kan JAL. AFLP analysis of genetic diversity in populations of Botrytis elliptica and Botrytis tulipae from the Netherlands. Eur J Plant Pathol 117: 219-235, 2007. Staats M, van Baarlen P, van Kan JAL. Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Mol Biol Evol 22: 333-346, 2005. PubMed: 15496556 Sahm H, et al. Purification and characterization of four extracellular 1,3-beta-glucanases of Botrytis cinerea. J. Gen. Microbiol. 139: 2833-2840, 1993. Faretra F, Pollastro S. Isolation, characterization and genetic analysis of laboratory mutants of Botryotinia fuckeliana resistant to the phenylpyrrole fungicide CGA 173506. Mycol. Res. 97: 620-624, 1993. Faretra F, Pollastro S. Genetics of sexual compatibility and resistance to benzimidazole and dicarboximide fungicides in isolates of Botryotinia fuckeliana (Botrytis cinerea) from nine countries. Plant Pathol. 42: 48-57, 1993. Faretra F, Pollastro S. Genetic characterization of Botryotinia fuckeliana (Botrytis cinerea) field isolates coupling high resistance to benzimidazoles to insensitivity toward the N-phenylcarbamate diethofencarb (*). Phytopathol. Mediterr. 31: 148-153, 1992. Faretra F, Pollastro S. Genetic basis of resistance to benzimidazole and dicarboximide fungicides in Botryotinia fuckeliana (Botrytis cinerea). Mycol. Res. 95: 943-951, 1991. Faretra F, et al. Sexual behaviour and mating system of Botryotinia fuckeliana, teleomorph of Botrytis cinerea. J. Gen. Microbiol. 134: 2543-2550, 1988. Faretra F, Pollastro S. Effect of the substrate on the formation of sclerotia of Botryotinia fuckeliana (Botrytis cinerea) and on their ability to germinate carpogenically. Phytopathol. Mediterr. 27: 99-102, 1988. |
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| 产品名称： | Botryotinia fuckeliana (de Bary) Whetzel, teleomorph |
|---|---|
| 商品货号： | TS201589 |
| Deposited As： | Botryotinia fuckeliana (de Bary) Whetzel |
| Strain Designations： | SAS405 |
| Alternate State： | Botrytis cinerea Persoon, anamorph |
| Application： | Produces glucan 1,3-beta-glucosidase beta-D-1,3-glucanase, exo-1,3-beta-glucanase, exo-1,3-beta-glucosidase Produces extracellular 1,3-beta-glucanases |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Genotype： | MAT1-2 Mbc1HR Daf1LR |
| Preceptrol®： | no |
| Ploidy： | haploid |
| Comments： | Mating system
Growth in culture
Genetics of fungicide resistance |
| Morphology： | Colonies at 25°C on Potato sucrose agar after 4-5 days are greyish brown and sclerotia black small. Conidia ellipsoidal and smooth. Place culture under UV light for sporulation. |
| Medium： | ATCC® Medium 324: Malt extract agar ATCC® Medium 338: Potato sucrose agar |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence TCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTCATGCCCGAAAGGGTAGACCTCCCACCCTTGTGTATTATTACTTTGTTGCTTTGGCGAGCTGCCTTCGGGCCTTGTATGCTCGCCAGAGAATACCAAAACTCTTTTTATTAATGTCGTCTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCTTAGCTTGGTATTGAGTCTATGTCAGTAATGGCAGGCTCTAAAATCAGTGGCGGCGCCGCTGGGTCCTGAACGTAGTAATATCTCTCGTTACAGGTTCTCGGTGTGCTTCTGCCAAAACCCAAATTTTTCTATGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTACCTCAGTAACGGCGAGTGAAGCGGTAAAAGCTCAAATTTGAAATCTGGCTCTTTTAGAGTCCGAATTGTAATTTGTAGAAGATGCTTCGGGTGTGGTTCCGGTCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGTGACTGGATACCTATGCTCATGTGAAGCTCTTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGAGGTATATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGCAATCAGACTTGCACTTGGTGTTCATCAGGGTCTCGTACCCTGTGTACTTCATCAAGTTCAGGCCAGCATCAGTTTGAGTGGTTAGATAAAGGCTTAGAGAATGTGGCCCTCTTCGGGGGGTGTTATAGCTCTAGGTGCAATGTAGCCTACTTGGACTGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGAC |
| Name of Depositor： | F Faretra |
| Isolation： | Single-ascospore isolate |
| Cross References： | Nucleotide (GenBank) : KP780449 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KP780471 D1/D2 region of 28S rRNA |
| References： | Staats M, et al. Positive selection in phytotoxic protein-encoding genes of Botrytis species. Fungal Genet Biol 44: 52-63, 2007. PubMed: 16935013 Staats M, van Baarlen P, van Kan JAL. AFLP analysis of genetic diversity in populations of Botrytis elliptica and Botrytis tulipae from the Netherlands. Eur J Plant Pathol 117: 219-235, 2007. Staats M, van Baarlen P, van Kan JAL. Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity. Mol Biol Evol 22: 333-346, 2005. PubMed: 15496556 Sahm H, et al. Purification and characterization of four extracellular 1,3-beta-glucanases of Botrytis cinerea. J. Gen. Microbiol. 139: 2833-2840, 1993. Faretra F, Pollastro S. Isolation, characterization and genetic analysis of laboratory mutants of Botryotinia fuckeliana resistant to the phenylpyrrole fungicide CGA 173506. Mycol. Res. 97: 620-624, 1993. Faretra F, Pollastro S. Genetics of sexual compatibility and resistance to benzimidazole and dicarboximide fungicides in isolates of Botryotinia fuckeliana (Botrytis cinerea) from nine countries. Plant Pathol. 42: 48-57, 1993. Faretra F, Pollastro S. Genetic characterization of Botryotinia fuckeliana (Botrytis cinerea) field isolates coupling high resistance to benzimidazoles to insensitivity toward the N-phenylcarbamate diethofencarb (*). Phytopathol. Mediterr. 31: 148-153, 1992. Faretra F, Pollastro S. Genetic basis of resistance to benzimidazole and dicarboximide fungicides in Botryotinia fuckeliana (Botrytis cinerea). Mycol. Res. 95: 943-951, 1991. Faretra F, et al. Sexual behaviour and mating system of Botryotinia fuckeliana, teleomorph of Botrytis cinerea. J. Gen. Microbiol. 134: 2543-2550, 1988. Faretra F, Pollastro S. Effect of the substrate on the formation of sclerotia of Botryotinia fuckeliana (Botrytis cinerea) and on their ability to germinate carpogenically. Phytopathol. Mediterr. 27: 99-102, 1988. |
