SMH-5
SMH-5
规格:
货期:
编号:TS201718
品牌:Testobio
| 产品名称： | SMH-5 |
|---|---|
| 商品货号： | TS201718 |
| Designations： | SMH-5 |
| GenBank Number： | X83618 |
| Species： | Homo sapiens, human |
| Depositors： | FG Hegardt |
| Vector： | Construct size (kb): 5.019999980926514 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HMGCS2 Gene name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 p13-p12 Tissue: liver Type of DNA: cDNA Insert end: Modification: EcoRI Insert end: Modification: EcoRI Insert size (kb): 2.058 Cross references: DNA Seq. Acc.: X83618 Nucleotides 1-2058 of the insert correspond to nucleotides 1-2058 of X83618. Insert lengths(kb): 2.058000087738037 Tissue: liver Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) HMGCS2 |
| Insert Size (kb)： | 2.058 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; HindIII--3.2, 0.9, 0.9; KpnI--3.2, 1.8; PstI--4.4, 0.55; StuI--5.0. The insert contains the following restriciton sites (approximate kb from the 5 end): HindIII--0.82,1.60; StuI--1.12; KpnI--1.52; PstI--1.59. Use of BLAST shows that 99% (2468/1476) of nt 485-1960 in X83618 are identical to nt 1-1474 of U12789. |
| References： | Mascaro C, et al. Molecular cloning and tissue expression of human mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase. Arch. Biochem. Biophys. 317: 385-390, 1995. PubMed: 7893153 |
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13372109301
| 产品名称： | SMH-5 |
|---|---|
| 商品货号： | TS201718 |
| Designations： | SMH-5 |
| GenBank Number： | X83618 |
| Species： | Homo sapiens, human |
| Depositors： | FG Hegardt |
| Vector： | Construct size (kb): 5.019999980926514 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: Polylinker sites: Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HMGCS2 Gene name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 p13-p12 Tissue: liver Type of DNA: cDNA Insert end: Modification: EcoRI Insert end: Modification: EcoRI Insert size (kb): 2.058 Cross references: DNA Seq. Acc.: X83618 Nucleotides 1-2058 of the insert correspond to nucleotides 1-2058 of X83618. Insert lengths(kb): 2.058000087738037 Tissue: liver Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) HMGCS2 |
| Insert Size (kb)： | 2.058 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; HindIII--3.2, 0.9, 0.9; KpnI--3.2, 1.8; PstI--4.4, 0.55; StuI--5.0. The insert contains the following restriciton sites (approximate kb from the 5 end): HindIII--0.82,1.60; StuI--1.12; KpnI--1.52; PstI--1.59. Use of BLAST shows that 99% (2468/1476) of nt 485-1960 in X83618 are identical to nt 1-1474 of U12789. |
| References： | Mascaro C, et al. Molecular cloning and tissue expression of human mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase. Arch. Biochem. Biophys. 317: 385-390, 1995. PubMed: 7893153 |
