VMM1

规格:

货期:

编号:TS201844

品牌:Testobio

产品名称：	VMM1
商品货号：	TS201844
Organism：	Homo sapiens, human
Tissue：	Melanoma, brain metastasis
Cell Type：	Melanocyte
Product Format：	frozen
Morphology：	Epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Melanoma, Stage IV; malignant
Age：	62
Gender：	Male
Ethnicity：	Caucasian
Applications：	Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Derived from a metastatic tumor in the brain surgically resected from a patient at University of Virginia
Clinical Data：	Primary Site: Unknown; Metastatic Site: Brain
Antigen Expression：	Positives: GP100, Tyrosinase, MAGE-A1
HLA Typing：	A3, A26/10(A26), B60(B40), B51/52(B*51), Cw6, Cw7
Comments：	NRAS Mutation: Q61L Q61R CDKN2A Mutation: R80 (One allele, (wt or unknown for the other)) BRAF wt
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37^oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the xa0 flask. Check the xa0cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach confluence.
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂ ), 5%
STR Profile：	Amelogenin: X,Yxa0 D5S818: 9xa0 D7S820: 9,12xa0 D13S317: 9,11xa0 D16S539:10 xa0 vWA: 16,18 xa0 TH01: 10,10xa0 TPOX: 8xa0 CSF1PO: 11 xa0
Sterility Tests：	Pass
Name of Depositor：	Craig L. Slingluff, Jr. M.D.
Passage History：	Unknown. 3-4 passages from 1997 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1991.
Year of Origin：	1991
References：	Slingluff C, et al. Melanomas with concordant loss of multiple melanocytic differentiation proteins: immune escape that may be overcome by targeting unique or undefined antigens. Cancer Immunol. Immunother. 48:661-672, 2000. PubMed: 10752474 Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344 Molhoek K, et al. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and Vascular Endothelial Growth Factor/Vascular Endothelial Growth Factor Receptor-2. Cancer Res. 68: (11), 2008. PubMed: 18519701 Skipper J, et al. Shared epitopes for HLA-A3 = restricted melanoma-reactive hman CTL include a naturally processed epitope from Pmel-I 7/gp100. J. Immunol. 157: 5027-5033, 1996. PubMed: 8943411

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VMM1

货号: TS201844
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产品名称：	VMM1
商品货号：	TS201844
Organism：	Homo sapiens, human
Tissue：	Melanoma, brain metastasis
Cell Type：	Melanocyte
Product Format：	frozen
Morphology：	Epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Melanoma, Stage IV; malignant
Age：	62
Gender：	Male
Ethnicity：	Caucasian
Applications：	Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Derived from a metastatic tumor in the brain surgically resected from a patient at University of Virginia
Clinical Data：	Primary Site: Unknown; Metastatic Site: Brain
Antigen Expression：	Positives: GP100, Tyrosinase, MAGE-A1
HLA Typing：	A3, A26/10(A26), B60(B40), B51/52(B*51), Cw6, Cw7
Comments：	NRAS Mutation: Q61L Q61R CDKN2A Mutation: R80 (One allele, (wt or unknown for the other)) BRAF wt
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37^oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the xa0 flask. Check the xa0cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach confluence.
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂ ), 5%
STR Profile：	Amelogenin: X,Yxa0 D5S818: 9xa0 D7S820: 9,12xa0 D13S317: 9,11xa0 D16S539:10 xa0 vWA: 16,18 xa0 TH01: 10,10xa0 TPOX: 8xa0 CSF1PO: 11 xa0
Sterility Tests：	Pass
Name of Depositor：	Craig L. Slingluff, Jr. M.D.
Passage History：	Unknown. 3-4 passages from 1997 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1991.
Year of Origin：	1991
References：	Slingluff C, et al. Melanomas with concordant loss of multiple melanocytic differentiation proteins: immune escape that may be overcome by targeting unique or undefined antigens. Cancer Immunol. Immunother. 48:661-672, 2000. PubMed: 10752474 Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344 Molhoek K, et al. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and Vascular Endothelial Growth Factor/Vascular Endothelial Growth Factor Receptor-2. Cancer Res. 68: (11), 2008. PubMed: 18519701 Skipper J, et al. Shared epitopes for HLA-A3 = restricted melanoma-reactive hman CTL include a naturally processed epitope from Pmel-I 7/gp100. J. Immunol. 157: 5027-5033, 1996. PubMed: 8943411