| 产品名称： | fd-tet |
|---|---|
| 商品货号： | TS202401 |
| Designations： | fd-tet |
| Depositors： | GP Smith |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.2 Vector: fd-tet (phage, lambda - replacement) Construction: phage fd, Tn10 Marker(s):tetR Construct size (kb): 9.2 Features: marker(s): tetR replicon: fd |
| Applications： | vector permitting production of single-stranded DNA |
| Comments： | Distributed as infected E. coli JM101 (ATCC 33876). Has been used as the starting material to construct a vector expressing immunoglobulin variable region-gene III fusion proteins which can be screened by antigen with the intact bacteriophage ("phage antibodies"). |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Zacher AN, et al. A new filamentous phage cloning vector: fd-tet. Gene 9: 127-140, 1980. PubMed: 6247241 Nelson FK, et al. Filamentous phage DNA cloning vectors: a noninfective mutant with a nonpolar deletion in gene III. Virology 108: 338-350, 1981. PubMed: 6258292 McCafferty J, et al. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348: 552-554, 1990. PubMed: 2247164 |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of fd-tet infected E. coli |
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| 产品名称： | fd-tet |
|---|---|
| 商品货号： | TS202401 |
| Designations： | fd-tet |
| Depositors： | GP Smith |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.2 Vector: fd-tet (phage, lambda - replacement) Construction: phage fd, Tn10 Marker(s):tetR Construct size (kb): 9.2 Features: marker(s): tetR replicon: fd |
| Applications： | vector permitting production of single-stranded DNA |
| Comments： | Distributed as infected E. coli JM101 (ATCC 33876). Has been used as the starting material to construct a vector expressing immunoglobulin variable region-gene III fusion proteins which can be screened by antigen with the intact bacteriophage ("phage antibodies"). |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Zacher AN, et al. A new filamentous phage cloning vector: fd-tet. Gene 9: 127-140, 1980. PubMed: 6247241 Nelson FK, et al. Filamentous phage DNA cloning vectors: a noninfective mutant with a nonpolar deletion in gene III. Virology 108: 338-350, 1981. PubMed: 6258292 McCafferty J, et al. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348: 552-554, 1990. PubMed: 2247164 |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of fd-tet infected E. coli |
