HO-poly-KanMX4-HO
HO-poly-KanMX4-HO
规格:
货期:
编号:TS202598
品牌:Testobio
| 产品名称： | HO-poly-KanMX4-HO |
|---|---|
| 商品货号： | TS202598 |
| Designations： | HO-poly-KanMX4-HO |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Construct size (kb): 6.06
Vector: HO-poly-KanMX4-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Marker(s): ampR
Features: promoter: HO |
| Applications： | Cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--3.0 (doublet); XbaI--6.0; PstI--5.8, 0.2. The plasmid contains two fragments from the HO promoter inserted in pUC21-NotI polylinker along with the kanMX selectable marker. It allows for targeted integration at HO locus. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | David J Stillman, personal communication |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of E. coli containing the plasmid. |
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| 产品名称： | HO-poly-KanMX4-HO |
|---|---|
| 商品货号： | TS202598 |
| Designations： | HO-poly-KanMX4-HO |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Construct size (kb): 6.06
Vector: HO-poly-KanMX4-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Marker(s): ampR
Features: promoter: HO |
| Applications： | Cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): NotI--3.0 (doublet); XbaI--6.0; PstI--5.8, 0.2. The plasmid contains two fragments from the HO promoter inserted in pUC21-NotI polylinker along with the kanMX selectable marker. It allows for targeted integration at HO locus. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | David J Stillman, personal communication |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of E. coli containing the plasmid. |
