| 产品名称： | pT223 D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223 |
|---|---|
| 商品货号： | TS202617 |
| Designations： | pT223 D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223 |
| GenBank Number： | M11722 |
| Species： | Homo sapiens, human |
| Depositors： | Henry M. Jackson Foundation, FJ Bollum, Henry M. Jackson Foundation |
| Applications： | produces protein terminal deoxynucleotidyl transferase |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.736999988555908 Tissue: lymphoblast KM-3 cell line Gene product: terminal deoxynucleotidyl transferase TDT Target Gene: terminal deoxynucleotidyl transferase |
| Insert Size (kb)： | 1.737 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; PvuII--2.3, 1.1, 0.6, 0.4; BamHI/EcoRI--2.6, 1.1, 0.7; HindIII--4.4; BglII--4.4. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.64; XmnI--0.17, 0.63, 0.79; PvuII--0.9, 1.25. The insert corresponds to nt 332-2068 of the published sequence, including all of the CDS (nt 329-2068) except for the initiation ATG. The recombinant fusion protein lacks the human N-terminal methionine and includes 15 amino acids encoded by the vector. It is immunoreactive and demonstrates catalytic activity. |
| References： | Peterson RC, et al. Molecular cloning of human terminal deoxynucleotidyltransferase. Proc. Natl. Acad. Sci. USA 81: 4363-4367, 1984. PubMed: 6087320 Peterson RC, et al. Expression of human terminal deoxynucleotidyl transferase in Escherichia coli. J. Biol. Chem. 260: 10495-10502, 1985. PubMed: 2863268 Bollum FJ, et al. Recombinant DNA molecules for producing terminal transferase-like polypeptides. US Patent 5,037,756 dated Aug 6 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pT223 D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223 |
|---|---|
| 商品货号： | TS202617 |
| Designations： | pT223 D10S2140, UTR-9747, pUC19(BamHI-EcoRI),pT223 |
| GenBank Number： | M11722 |
| Species： | Homo sapiens, human |
| Depositors： | Henry M. Jackson Foundation, FJ Bollum, Henry M. Jackson Foundation |
| Applications： | produces protein terminal deoxynucleotidyl transferase |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.736999988555908 Tissue: lymphoblast KM-3 cell line Gene product: terminal deoxynucleotidyl transferase TDT Target Gene: terminal deoxynucleotidyl transferase |
| Insert Size (kb)： | 1.737 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; PvuII--2.3, 1.1, 0.6, 0.4; BamHI/EcoRI--2.6, 1.1, 0.7; HindIII--4.4; BglII--4.4. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.64; XmnI--0.17, 0.63, 0.79; PvuII--0.9, 1.25. The insert corresponds to nt 332-2068 of the published sequence, including all of the CDS (nt 329-2068) except for the initiation ATG. The recombinant fusion protein lacks the human N-terminal methionine and includes 15 amino acids encoded by the vector. It is immunoreactive and demonstrates catalytic activity. |
| References： | Peterson RC, et al. Molecular cloning of human terminal deoxynucleotidyltransferase. Proc. Natl. Acad. Sci. USA 81: 4363-4367, 1984. PubMed: 6087320 Peterson RC, et al. Expression of human terminal deoxynucleotidyl transferase in Escherichia coli. J. Biol. Chem. 260: 10495-10502, 1985. PubMed: 2863268 Bollum FJ, et al. Recombinant DNA molecules for producing terminal transferase-like polypeptides. US Patent 5,037,756 dated Aug 6 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
