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Trypanosoma lewisi (Kent) Laveran and Mesnil
Trypanosoma lewisi (Kent) Laveran and Mesnil
规格:
货期:
编号:TS202624
品牌:Testobio
产品名称： Trypanosoma lewisi (Kent) Laveran and Mesnil
商品货号： TS202624
Strain Designations： New Orleans-67
Application：
Vector borne research
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
rat, Rattus norvegicus, New Orleans, 1967
Product Format： frozen
Type Strain： no
Medium： ATCC® Medium 1029: LIT medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation：
  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mL ATCC medium 1029 supplemented with an additional 5-10 μg/mL hemin.xa0
  10. Incubate at 25°C with the cap screwed on tightly.
  11. Maintain as described above. xa0
Name of Depositor： RG Yaeger
Year of Origin： 1967
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Trypanosoma lewisi (Kent) Laveran and Mesnil

  • 货号: TS202624
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Trypanosoma lewisi (Kent) Laveran and Mesnil
商品货号： TS202624
Strain Designations： New Orleans-67
Application：
Vector borne research
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
rat, Rattus norvegicus, New Orleans, 1967
Product Format： frozen
Type Strain： no
Medium： ATCC® Medium 1029: LIT medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation：
  1. Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed.
  9. Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mL ATCC medium 1029 supplemented with an additional 5-10 μg/mL hemin.xa0
  10. Incubate at 25°C with the cap screwed on tightly.
  11. Maintain as described above. xa0
Name of Depositor： RG Yaeger
Year of Origin： 1967
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