| 产品名称： | Gibbon ape leukemia virus |
|---|---|
| 商品货号： | TS202670 |
| Classification： | Retroviridae, Gammaretrovirus |
| Agent： | Gibbon ape leukemia virus |
| Strain： | SEATO |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Comments： | Cells contain a GALV plasmid (pGEM13386) and are not infected with a primary infectious GALV. Cells are not G418 resistant. Cells should be monitored by RT-PCR to confirm that viral genes are expressed. At the time of deposit, this line was considered important as a source of early passage GALV SEATO strain, which was considered a reference material of utility to PG13 gene therapy researchers.xa0 |
| Effect on Host： | No CPE, RT-PCR to assess for virus presence |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection:xa0Thaw the cells in a 37°C water bath until the ice is just melted, then add 0.5 mL of cellsxa0directly to a T-25 flask containing fresh medium. Post-freeze recovery is slow. Perform complete media renewal on Day 3 and continue media renewal every 3 - 4 days. Monitor the cells for adherence and expect populations of cells to project epithelial structures on approximately Day 4 - Day 8. Cell growth rate significantly increases with subsequent subcultures.xa0 Incubation: 3-5 days, until confluent and passaging or harvesting is needed |
| Cryopreservation： | Freeze Medium: DMEM (ATCC® 30-2002™) + 10% FBS (ATCC® 30-2020™) + 7% DMSO (ATCC® 4-X™) |
| Name of Depositor： | MV Eiden, National Institute of Mental Health |
| Special Collection： | NCRR Contract |
| Source： | Mink cells expressing Gibbon ape leukemia virus (GALV). These infected cells were produced by transfecting a clone of GALV SEATO strain into mink cells and testing cell supernatant after several passages for a sustained peak in reverse transcriptase activity. The clone was derived from sequences obtained from GALV San Francisco strain and SEATO strain. |
| References： | also affects subhuman primates and monkey cells Mink cells expressing Gibbon Ape Leukemia Virus. These infected cells were produced by transfecting a biologically clone of GALV-SEATO into mink cells and testing cell supernatant after several passages for a sustained peak in reverse transcriptase activity-designating that the cells are productively infected. The GALV clone was derived from sequences obtained from GALV strain San Francisco and SEATO, pGEM-13-386, GaLV-SEATO. |
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| 产品名称： | Gibbon ape leukemia virus |
|---|---|
| 商品货号： | TS202670 |
| Classification： | Retroviridae, Gammaretrovirus |
| Agent： | Gibbon ape leukemia virus |
| Strain： | SEATO |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | Vapor phase of liquid nitrogen |
| Comments： | Cells contain a GALV plasmid (pGEM13386) and are not infected with a primary infectious GALV. Cells are not G418 resistant. Cells should be monitored by RT-PCR to confirm that viral genes are expressed. At the time of deposit, this line was considered important as a source of early passage GALV SEATO strain, which was considered a reference material of utility to PG13 gene therapy researchers.xa0 |
| Effect on Host： | No CPE, RT-PCR to assess for virus presence |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection:xa0Thaw the cells in a 37°C water bath until the ice is just melted, then add 0.5 mL of cellsxa0directly to a T-25 flask containing fresh medium. Post-freeze recovery is slow. Perform complete media renewal on Day 3 and continue media renewal every 3 - 4 days. Monitor the cells for adherence and expect populations of cells to project epithelial structures on approximately Day 4 - Day 8. Cell growth rate significantly increases with subsequent subcultures.xa0 Incubation: 3-5 days, until confluent and passaging or harvesting is needed |
| Cryopreservation： | Freeze Medium: DMEM (ATCC® 30-2002™) + 10% FBS (ATCC® 30-2020™) + 7% DMSO (ATCC® 4-X™) |
| Name of Depositor： | MV Eiden, National Institute of Mental Health |
| Special Collection： | NCRR Contract |
| Source： | Mink cells expressing Gibbon ape leukemia virus (GALV). These infected cells were produced by transfecting a clone of GALV SEATO strain into mink cells and testing cell supernatant after several passages for a sustained peak in reverse transcriptase activity. The clone was derived from sequences obtained from GALV San Francisco strain and SEATO strain. |
| References： | also affects subhuman primates and monkey cells Mink cells expressing Gibbon Ape Leukemia Virus. These infected cells were produced by transfecting a biologically clone of GALV-SEATO into mink cells and testing cell supernatant after several passages for a sustained peak in reverse transcriptase activity-designating that the cells are productively infected. The GALV clone was derived from sequences obtained from GALV strain San Francisco and SEATO, pGEM-13-386, GaLV-SEATO. |
