你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: call_new 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
Euplotes raikovi
Euplotes raikovi
规格:
货期:
编号:TS202917
品牌:Testobio
产品名称： Euplotes raikovi
商品货号： TS202917
Strain Designations： strain No. 13
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
Shallow interstitial seawater, Porto Recanati, Italy, 1979
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Xenic, Grows in the presence of Klebsiella pneumoniae subsp pneumoniae (ATCC 700831) or Enterobacter aerogenes (ATCC 13048) as food source
Type Strain： no
Comments：
Secretes water soluble protein pheromones Er-1 and Er-2
Medium： ATCC® Medium 1525: Seawater 802 medium
ATCC® Medium 1405: HESNW medium
Growth Conditions：
Temperature: 20°C
Cryopreservation：

Cryoprotective Solution
xa0xa0xa0 DMSO, 2.0 ml
xa0xa0xa0 Fresh growth medium w/o bacteria, 8.0 ml

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that has recently passed peak density by centrifugation at 1200-1600 x g for 10 min.
  3. Adjust the concentration of cysts at least 2 x 105/ml in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831™) or Enterobacter aerogenes (ATCC® 13048™).
  9. Incubate the culture at 25°C with the cap screwed on tightly.

    Once the culture is established, follow the protocol for maintenance of culture.
Name of Depositor： P Luporini
Year of Origin： 1979
References：

Miceli C, et al. Identification and structural characterization of a cDNA clone encoding a membrane-bound form of the polypeptide pheromone Er-1 in the ciliate protozoan Euplotes raikovi. Proc. Natl. Acad. Sci. USA 89: 1988-1992, 1992. PubMed: 1542697

Vallesi A, et al. A novel protein kinase from the ciliate Euplotes raikovi with close structural identity to the mammalian intestinal and male-germ cell kinases: characterization and functional implications in the autocrine pheromone signaling loop. Protist 161: 250-63, 2010. PubMed: 20075005

首页 > 产品中心 > 微生物培养 > 菌株 > null > Euplotes raikovi

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

Euplotes raikovi

  • 货号: TS202917
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Euplotes raikovi
商品货号： TS202917
Strain Designations： strain No. 13
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
Shallow interstitial seawater, Porto Recanati, Italy, 1979
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Xenic, Grows in the presence of Klebsiella pneumoniae subsp pneumoniae (ATCC 700831) or Enterobacter aerogenes (ATCC 13048) as food source
Type Strain： no
Comments：
Secretes water soluble protein pheromones Er-1 and Er-2
Medium： ATCC® Medium 1525: Seawater 802 medium
ATCC® Medium 1405: HESNW medium
Growth Conditions：
Temperature: 20°C
Cryopreservation：

Cryoprotective Solution
xa0xa0xa0 DMSO, 2.0 ml
xa0xa0xa0 Fresh growth medium w/o bacteria, 8.0 ml

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that has recently passed peak density by centrifugation at 1200-1600 x g for 10 min.
  3. Adjust the concentration of cysts at least 2 x 105/ml in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831™) or Enterobacter aerogenes (ATCC® 13048™).
  9. Incubate the culture at 25°C with the cap screwed on tightly.

    Once the culture is established, follow the protocol for maintenance of culture.
Name of Depositor： P Luporini
Year of Origin： 1979
References：

Miceli C, et al. Identification and structural characterization of a cDNA clone encoding a membrane-bound form of the polypeptide pheromone Er-1 in the ciliate protozoan Euplotes raikovi. Proc. Natl. Acad. Sci. USA 89: 1988-1992, 1992. PubMed: 1542697

Vallesi A, et al. A novel protein kinase from the ciliate Euplotes raikovi with close structural identity to the mammalian intestinal and male-germ cell kinases: characterization and functional implications in the autocrine pheromone signaling loop. Protist 161: 250-63, 2010. PubMed: 20075005

testopic
小红书
扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: