pGL2BIFN
pGL2BIFN
规格:
货期:
编号:TS203201
品牌:Testobio
| 产品名称： | pGL2BIFN |
|---|---|
| 商品货号： | TS203201 |
| Designations： | pGL2BIFN |
| Species： | Homo sapiens, human |
| Depositors： | Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V. |
| Applications： | in another host, produces protein interferon, alpha 2 |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGL2B Intact vector size: 6.600 Type of vector: plasmid Cloning sites: EcoRV, BglII Polylinker sites: Other unique sites: BamHI, HindIII Construction: pUC12 Host range: Escherichia coli; filamentous fungi Features (with orientation and position when available): marker(s): ampR replicon: pMB1 promoter: glucoamylase, -> signal peptide coding region: glucoamylase, -> Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNA2 Genomic copy number: unique Gene name: interferon, alpha 2 Contains complete coding sequence?: Y Chromosome: 9; Localization: 9 p22 Type of DNA: cDNA Insert 5 end: DdeI Modification: filled Insert 3 end: BamHI Modification: filled Insert size (kb): Cross references: Gene product: interferon, alpha 2 IFNA2 Target Gene: interferon, alpha 2 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 1.9, 1.2; BamHI--8.0. The construct has been demonstrated to direct secretion of protein after starch induction in both A. niger and A. nidulans. Should be cotransformed with a plasmid expressing argB for selection. |
| References： | Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pGL2BIFN |
|---|---|
| 商品货号： | TS203201 |
| Designations： | pGL2BIFN |
| Species： | Homo sapiens, human |
| Depositors： | Gist-Brocades N.V., DI Gwynne, Gist-Brocades N.V. |
| Applications： | in another host, produces protein interferon, alpha 2 |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGL2B Intact vector size: 6.600 Type of vector: plasmid Cloning sites: EcoRV, BglII Polylinker sites: Other unique sites: BamHI, HindIII Construction: pUC12 Host range: Escherichia coli; filamentous fungi Features (with orientation and position when available): marker(s): ampR replicon: pMB1 promoter: glucoamylase, -> signal peptide coding region: glucoamylase, -> Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: IFNA2 Genomic copy number: unique Gene name: interferon, alpha 2 Contains complete coding sequence?: Y Chromosome: 9; Localization: 9 p22 Type of DNA: cDNA Insert 5 end: DdeI Modification: filled Insert 3 end: BamHI Modification: filled Insert size (kb): Cross references: Gene product: interferon, alpha 2 IFNA2 Target Gene: interferon, alpha 2 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 1.9, 1.2; BamHI--8.0. The construct has been demonstrated to direct secretion of protein after starch induction in both A. niger and A. nidulans. Should be cotransformed with a plasmid expressing argB for selection. |
| References： | Gwynne DI, et al. Vectors in use in filamentous fungi. US Patent 5,198,345 dated Mar 30 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
