| 产品名称： | Mammalian orthoreovirus 1 |
|---|---|
| 商品货号： | TS203235 |
| Classification： | Reoviridae, Orthoreovirus |
| Deposited As： | Reovirus type 1 |
| Agent： | Mammalian orthoreovirus 1 |
| Strain： | Lang |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Rectal swab from healthy child, Ohio, 1953 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | For passage cell cultures should be harvested after complete involvement of cell sheet. Culture fluid contains a group-specific CF antigen. REFERENCE STRAIN: Sabin, A.B., Science (Washington, DC) 130: 1387, 1959. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects (granulated degeneration of cells and then destruction of entire cell sheet) CPE; granulated degeneration of cells and then destruction of entire cell sheet |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host: LLC-MK2 (ATCC CCL-7.1) |
| Growth Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Duration: 2-6 days |
| Name of Depositor： | EH Lennette |
| Source： | Rectal swab from healthy child, Ohio, 1953 |
| Year of Origin： | 1953 |
| References： | Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336 Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578 |
| Cross References： | Nucleotide (GenBank) : AJ133089 Reovirus type 1 S2 gene, partial, strain Lang. Nucleotide (GenBank) : M13139 Reovirus (Lang strain) s4 mRNA encoding the major capsid surface protein sigma-3, complete cds. Nucleotide (GenBank) : M14325 Reovirus (Lang strain) s3 gene encoding the nonstructural RNA-binding protein sigma-NS, complete cds. Nucleotide (GenBank) : M14779 Reovirus (Lang strain) s1 mRNA encoding the minor capsid cell attachment protein sigma-1a and the nonstructural protein sigma-ibNS, complete cds. |
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| 产品名称： | Mammalian orthoreovirus 1 |
|---|---|
| 商品货号： | TS203235 |
| Classification： | Reoviridae, Orthoreovirus |
| Deposited As： | Reovirus type 1 |
| Agent： | Mammalian orthoreovirus 1 |
| Strain： | Lang |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Rectal swab from healthy child, Ohio, 1953 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | For passage cell cultures should be harvested after complete involvement of cell sheet. Culture fluid contains a group-specific CF antigen. REFERENCE STRAIN: Sabin, A.B., Science (Washington, DC) 130: 1387, 1959. |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects (granulated degeneration of cells and then destruction of entire cell sheet) CPE; granulated degeneration of cells and then destruction of entire cell sheet |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host: LLC-MK2 (ATCC CCL-7.1) |
| Growth Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Duration: 2-6 days |
| Name of Depositor： | EH Lennette |
| Source： | Rectal swab from healthy child, Ohio, 1953 |
| Year of Origin： | 1953 |
| References： | Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336 Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578 |
| Cross References： | Nucleotide (GenBank) : AJ133089 Reovirus type 1 S2 gene, partial, strain Lang. Nucleotide (GenBank) : M13139 Reovirus (Lang strain) s4 mRNA encoding the major capsid surface protein sigma-3, complete cds. Nucleotide (GenBank) : M14325 Reovirus (Lang strain) s3 gene encoding the nonstructural RNA-binding protein sigma-NS, complete cds. Nucleotide (GenBank) : M14779 Reovirus (Lang strain) s1 mRNA encoding the minor capsid cell attachment protein sigma-1a and the nonstructural protein sigma-ibNS, complete cds. |
