| 产品名称： | neu c(t)/SP6400 |
|---|---|
| 商品货号： | TS203483 |
| Designations： | neu c(t)/SP6400 |
| Species： | Rattus norvegicus, rat |
| Depositors： | RA Weinberg, CI Bargmann |
| Vector： | Construct size (kb): 3.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 0.4199999868869782 Tissue: neuroblastoma B104 cell line Gene product: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)( Avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2, Neu oncogene, avian erythroblastosis oncogene B 2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogen, tyrosine kinase, receptor HER2) ERBB2 Alleles: b, s, |
| Insert Size (kb)： | 0.420 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.95, 0.42; HindIII--3.4. Insert contains StuI (2), BglII, and XhoI sites. Fragment derives from presumed extracellular portion of the gene. Deduced amino acid sequence is 40% homologous to EGF receptor. Does not hybridize to EGF receptor gene (c-erbB) even at relatively low stringency (30% formamide). Insert is oriented 3 to 5 relative to the SP6 promoter; transcription yields a probe complementary to neu mRNA. |
| References： | Bargmann CI, et al. The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature 319: 226-230, 1986. PubMed: 3945311 Bargmann CI, et al. Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Cell 45: 649-657, 1986. PubMed: 2871941 Cornelia I Bargmann, personal communication |
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| 产品名称： | neu c(t)/SP6400 |
|---|---|
| 商品货号： | TS203483 |
| Designations： | neu c(t)/SP6400 |
| Species： | Rattus norvegicus, rat |
| Depositors： | RA Weinberg, CI Bargmann |
| Vector： | Construct size (kb): 3.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 0.4199999868869782 Tissue: neuroblastoma B104 cell line Gene product: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)( Avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 2, Neu oncogene, avian erythroblastosis oncogene B 2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogen, tyrosine kinase, receptor HER2) ERBB2 Alleles: b, s, |
| Insert Size (kb)： | 0.420 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.95, 0.42; HindIII--3.4. Insert contains StuI (2), BglII, and XhoI sites. Fragment derives from presumed extracellular portion of the gene. Deduced amino acid sequence is 40% homologous to EGF receptor. Does not hybridize to EGF receptor gene (c-erbB) even at relatively low stringency (30% formamide). Insert is oriented 3 to 5 relative to the SP6 promoter; transcription yields a probe complementary to neu mRNA. |
| References： | Bargmann CI, et al. The neu oncogene encodes an epidermal growth factor receptor-related protein. Nature 319: 226-230, 1986. PubMed: 3945311 Bargmann CI, et al. Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Cell 45: 649-657, 1986. PubMed: 2871941 Cornelia I Bargmann, personal communication |
