| 产品名称： | Chlamydophila pecorum (Fukushi and Hirai) Everett et al. |
|---|---|
| 商品货号： | TS203492 |
| Classification： | Chlamydiaceae |
| Deposited As： | Chlamydia psittaci (Lillie) Page |
| Agent： | Chlamydophila pecorum (Fukushi and Hirai) Everett et al. |
| Strain： | Sporadic Bovine Encephalomyelitis strain E58 (McNutt) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Growth rate markedly stimulated by increases in incubation temperature. Optimum temperature for growth in CE is 39°C. Previously classified as C. psittaci; see Fukushi & Hirai (IJSB. 42: 306, 1992) for pecorum classification. FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | In TC, requires FA to visualize intracellular inclusion bodies. Lethal for CE with hyperemia and hemorrhage in y.s. and embryo |
| Recommended Host： | McCoy (ATCC CRL-1696) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent as the cylcoheximide will prevent further growth. Incubation: 48 to 72 hours, a 5%CO2 in air atmosphere is recommended.
|
| Name of Depositor： | LA Page |
| Source： | Calf brain from herd in Iowa, USA |
| References： | Fukushi H, Hirai K. Proposal of Chlamydia pecorum sp. nov. for Chlamydia strains derived from ruminants. Int. J. Syst. Bacteriol. 42: 306-308, 1992. PubMed: 1581191 McNutt SH. . Cornell Vet. 30: 437, 1940. |
| Cross References： | Nucleotide (GenBank) : AJ012173 Chlamydophila pecorum partial rnpB gene for RNase P RNA subunit. Nucleotide (GenBank) : U68433 Chlamydia pecorum E58 16S ribosomal RNA gene, partial sequence, intergenic spacer, and 23S ribosomal RNA gene, partial sequence. |
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| 产品名称： | Chlamydophila pecorum (Fukushi and Hirai) Everett et al. |
|---|---|
| 商品货号： | TS203492 |
| Classification： | Chlamydiaceae |
| Deposited As： | Chlamydia psittaci (Lillie) Page |
| Agent： | Chlamydophila pecorum (Fukushi and Hirai) Everett et al. |
| Strain： | Sporadic Bovine Encephalomyelitis strain E58 (McNutt) |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Growth rate markedly stimulated by increases in incubation temperature. Optimum temperature for growth in CE is 39°C. Previously classified as C. psittaci; see Fukushi & Hirai (IJSB. 42: 306, 1992) for pecorum classification. FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | In TC, requires FA to visualize intracellular inclusion bodies. Lethal for CE with hyperemia and hemorrhage in y.s. and embryo |
| Recommended Host： | McCoy (ATCC CRL-1696) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent as the cylcoheximide will prevent further growth. Incubation: 48 to 72 hours, a 5%CO2 in air atmosphere is recommended.
|
| Name of Depositor： | LA Page |
| Source： | Calf brain from herd in Iowa, USA |
| References： | Fukushi H, Hirai K. Proposal of Chlamydia pecorum sp. nov. for Chlamydia strains derived from ruminants. Int. J. Syst. Bacteriol. 42: 306-308, 1992. PubMed: 1581191 McNutt SH. . Cornell Vet. 30: 437, 1940. |
| Cross References： | Nucleotide (GenBank) : AJ012173 Chlamydophila pecorum partial rnpB gene for RNase P RNA subunit. Nucleotide (GenBank) : U68433 Chlamydia pecorum E58 16S ribosomal RNA gene, partial sequence, intergenic spacer, and 23S ribosomal RNA gene, partial sequence. |
