| 产品名称： | 2Q2 |
|---|---|
| 商品货号： | TS204394 |
| Designations： | 2Q2 |
| Species： | Homo sapiens, human |
| Depositors： | F Kao |
| Origin： | lymphoblast cell line GM03714 (46, XX) |
| Applications： | Colonies of different sizes may be observed. |
| Vector： | Tissue: lymphoblast cell line GM03714 (46, XX) Chromosome: 2q33-q35 DNA: genomic Digest: Complete Insert size range: 50-800 bp Coverage: Clones for "complete" coverage: 850000.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Colonies of different sizes may be observed. Region specific library constructed by microdissection of metaphase chromosomes, complete digestion with MboI, followed by PCR amplification and subcloning of amplification products. Approximately 94% of unique sequences were derived from chromosome 2, as determined by hybridization. Half of the clones contain repetitive sequences and all repetitive sequences are human and chromosome 2 specific. The library is estimated to be 97% pure. Approximately half of the clones in the library contain unique or low-copy number inserts. The mean insert size is 130 bp. |
| References： | Yu J, et al. Three region-specific microdissection libraries for the long arm of human chromosome 2, regions q33-q35, q31-q32 and q23-q24. Somatic Cell Mol. Genet. 21: 335-343, 1995. PubMed: 8619130 |
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| 产品名称： | 2Q2 |
|---|---|
| 商品货号： | TS204394 |
| Designations： | 2Q2 |
| Species： | Homo sapiens, human |
| Depositors： | F Kao |
| Origin： | lymphoblast cell line GM03714 (46, XX) |
| Applications： | Colonies of different sizes may be observed. |
| Vector： | Tissue: lymphoblast cell line GM03714 (46, XX) Chromosome: 2q33-q35 DNA: genomic Digest: Complete Insert size range: 50-800 bp Coverage: Clones for "complete" coverage: 850000.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Colonies of different sizes may be observed. Region specific library constructed by microdissection of metaphase chromosomes, complete digestion with MboI, followed by PCR amplification and subcloning of amplification products. Approximately 94% of unique sequences were derived from chromosome 2, as determined by hybridization. Half of the clones contain repetitive sequences and all repetitive sequences are human and chromosome 2 specific. The library is estimated to be 97% pure. Approximately half of the clones in the library contain unique or low-copy number inserts. The mean insert size is 130 bp. |
| References： | Yu J, et al. Three region-specific microdissection libraries for the long arm of human chromosome 2, regions q33-q35, q31-q32 and q23-q24. Somatic Cell Mol. Genet. 21: 335-343, 1995. PubMed: 8619130 |
