| 产品名称： | pbcr |
|---|---|
| 商品货号： | TS205290 |
| Designations： | pbcr |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.950000047683716 Gene product: breakpoint cluster region BCR Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 1.950 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.65, 1.95; HindIII--2.65, 1.95; PstI--2.65, 1.75; SalI--2.65, 1.95; EcoRI--4.5. This probe was originally assigned to D22S11. However, it has since been shown that D22S11 is within the BCR locus. Insert is 1.95 kb BglII/HindIII fragment containing exon 1 given in fig. 2b. The BglII site was lost by subcloning. The insert contains 2 TaqI sites; the polymorphic one is 0.5 kb from the HindIII end. Depositor verified that this preparation detects the TaqI RFLP. Enzyme(s) not detecting polymorphism: RsaI, PstI, EcoRI. Tested in 12 unrelated middle-Europeans. |
| References： | Roschmann E, et al. RFLP detected with a 5-bcr-gene-sequence HGM8 provisional no. D22S11. Nucleic Acids Res. 15: 1883, 1987. PubMed: 2881280 Heisterkamp N, et al. Structural organization of the bcr gene and its role in the Ph translocation. Nature 315: 758-761, 1985. PubMed: 2989703 Groffen J, et al. Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22. Cell 36: 93-99, 1984. PubMed: 6319012 Thomas Fink, personal communication |
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| 产品名称： | pbcr |
|---|---|
| 商品货号： | TS205290 |
| Designations： | pbcr |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.950000047683716 Gene product: breakpoint cluster region BCR Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 1.950 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.65, 1.95; HindIII--2.65, 1.95; PstI--2.65, 1.75; SalI--2.65, 1.95; EcoRI--4.5. This probe was originally assigned to D22S11. However, it has since been shown that D22S11 is within the BCR locus. Insert is 1.95 kb BglII/HindIII fragment containing exon 1 given in fig. 2b. The BglII site was lost by subcloning. The insert contains 2 TaqI sites; the polymorphic one is 0.5 kb from the HindIII end. Depositor verified that this preparation detects the TaqI RFLP. Enzyme(s) not detecting polymorphism: RsaI, PstI, EcoRI. Tested in 12 unrelated middle-Europeans. |
| References： | Roschmann E, et al. RFLP detected with a 5-bcr-gene-sequence HGM8 provisional no. D22S11. Nucleic Acids Res. 15: 1883, 1987. PubMed: 2881280 Heisterkamp N, et al. Structural organization of the bcr gene and its role in the Ph translocation. Nature 315: 758-761, 1985. PubMed: 2989703 Groffen J, et al. Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22. Cell 36: 93-99, 1984. PubMed: 6319012 Thomas Fink, personal communication |
