| 产品名称： | Saccharomyces kudriavzevii Naumov et al. |
|---|---|
| 商品货号： | TS209345 |
| Deposited As： | Saccharomyces cerevisiae |
| Strain Designations： | 2601-U |
| Application： | Assay of amphotericin B fungizone Assay of nystatin fungicidin Pharmaceutical and Personal Care |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | yes |
| Comments： | No vitamin requirement Reference strain for assays of amphotericin B fungizone and nystatin fungicidin designated by FDA. Ref  U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011The strain used to be classified as Saccharomyces cerevisiae based on traditional taxonomic characterizations, but a recent molecular authentication convincingly identified it as Saccharomyces kudriavzevii, not S. cerevisiae. The taxonomic update of this strain will not affect its application in antifungal drug testing assays and many other usages. Ref  Suh SO, et al. Update of USP drug testing reference strain ATCC® 2601™. Pharma. Microbiol. Forum Newslett. 19: 4-11, 2013. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25°C to 30°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internalxa0xa0 transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence;
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTGTTTGTTTTGGCAAGAGCGTGAGAGCTTTTACTGGGCAAGTAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGCAAGTTTCTTTCTTGCTATTCCAAACAGTGAGAGATTTCTCTGTTTTTGTTATAGGACAATCAAAACCGTTTCAATACAACACACTGTGGAGTTTTTATATCTTTGCAACTTTTTCTTTGGGCTGTCGAGCAATCGGAGCCCAGAGGTAACAAACACAAACAATTTTATTTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTTTCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTGTACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAATATTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAA D1D2 region of the 26S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGAACCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGCGCCCTCTGCTCCTTGTGGGTGGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCGTAGGAATGTAACTTGCTTCGGGAAGTATTATAGCCTGCGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGC |
| Name of Depositor： | NRRL |
| Chain of Custody： | ATCC <-- NRRL <-- FW Tanner <-- CBS 679 (Zygosaccharomyces priorianus) <-- A Guilliermond |
| Isolation： | Not available |
| Cross References： | Nucleotide (GenBank) : KC881068 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KC881065 D1/D2 region of 26S rRNA gene Nucleotide (GenBank) : KC881095 RNA polymerase II largest subunit (RPB1) gene Nucleotide (GenBank) : KC881092 polymerase II second largest subunit (RPB2) gene Nucleotide (GenBank) : KC881097 translation elongation factor 1-alpha gene Nucleotide (GenBank) : KC881100 mitochondrial small subunit ribosomal RNA gene |
| References： | Suh SO, et al. Update of USP drug testing reference strain ATCC® 2601™. Pharma. Microbiol. Forum Newslett. 19: 4-11, 2013. McGinnis MR. Laboratory handbook of medical mycology. New York: Academic Press; 1980. U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 |
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| 产品名称： | Saccharomyces kudriavzevii Naumov et al. |
|---|---|
| 商品货号： | TS209345 |
| Deposited As： | Saccharomyces cerevisiae |
| Strain Designations： | 2601-U |
| Application： | Assay of amphotericin B fungizone Assay of nystatin fungicidin Pharmaceutical and Personal Care |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | yes |
| Comments： | No vitamin requirement Reference strain for assays of amphotericin B fungizone and nystatin fungicidin designated by FDA. Ref U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011The strain used to be classified as Saccharomyces cerevisiae based on traditional taxonomic characterizations, but a recent molecular authentication convincingly identified it as Saccharomyces kudriavzevii, not S. cerevisiae. The taxonomic update of this strain will not affect its application in antifungal drug testing assays and many other usages. Ref Suh SO, et al. Update of USP drug testing reference strain ATCC® 2601™. Pharma. Microbiol. Forum Newslett. 19: 4-11, 2013. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25°C to 30°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internalxa0xa0 transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence;
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTGTTTGTTTTGGCAAGAGCGTGAGAGCTTTTACTGGGCAAGTAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGCAAGTTTCTTTCTTGCTATTCCAAACAGTGAGAGATTTCTCTGTTTTTGTTATAGGACAATCAAAACCGTTTCAATACAACACACTGTGGAGTTTTTATATCTTTGCAACTTTTTCTTTGGGCTGTCGAGCAATCGGAGCCCAGAGGTAACAAACACAAACAATTTTATTTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTTTCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTGTACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAATATTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAA D1D2 region of the 26S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGAACCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGCGCCCTCTGCTCCTTGTGGGTGGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCGTAGGAATGTAACTTGCTTCGGGAAGTATTATAGCCTGCGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGC |
| Name of Depositor： | NRRL |
| Chain of Custody： | ATCC <-- NRRL <-- FW Tanner <-- CBS 679 (Zygosaccharomyces priorianus) <-- A Guilliermond |
| Isolation： | Not available |
| Cross References： | Nucleotide (GenBank) : KC881068 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KC881065 D1/D2 region of 26S rRNA gene Nucleotide (GenBank) : KC881095 RNA polymerase II largest subunit (RPB1) gene Nucleotide (GenBank) : KC881092 polymerase II second largest subunit (RPB2) gene Nucleotide (GenBank) : KC881097 translation elongation factor 1-alpha gene Nucleotide (GenBank) : KC881100 mitochondrial small subunit ribosomal RNA gene |
| References： | Suh SO, et al. Update of USP drug testing reference strain ATCC® 2601™. Pharma. Microbiol. Forum Newslett. 19: 4-11, 2013. McGinnis MR. Laboratory handbook of medical mycology. New York: Academic Press; 1980. U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 |
