HFF-1

规格:

货期:

编号:TS209410

品牌:Testobio

产品名称：

HFF-1

商品货号：

TS209410

Organism：

Homo sapiens, human

Tissue：

Skin; foreskin

Cell Type：

Fibroblast

Product Format：

frozen

Morphology：

fibroblast

Culture Properties：

Adherent

Biosafety Level：

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease：

Normal

Age：

Newborn

Gender：

male

Applications：

Can be used to produce feeder cells

Storage Conditions：

Liquid nitrogen vapor phase

Derivation：

The cell line was established by ATCC in 2003 from normal human foreskin pooled from two individuals.

Clinical Data：

male

Comments：

The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated (SCRC-1041.1) and treated the cells with Mitomycin C (SCRC-1041.2) for use as a feeder layer. If the HFFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 50 (P50).xa0It is recommended that the feeder cells be plated 24 hours before use at 5X10^4xa0cells/cm² in order to obtain a supportive monolayer for stem cell growth.

Complete Growth Medium：

The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).

Subculturing：

Procedure:
To insure the highest level of viability, be sure to warm media and Trypsin/ EDTA to 37ºC before using it on the cells.

Cells should be split when they reach confluency. A split ratio of 1:5 to 1:7 is recommended. Volumes used in this protocol are for 225 cm² (T225); proportionally reduce or increase amount of dissociation medium for culture flasks of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 1X PBS (SCRR-2201) solution to remove all traces of serum, which contain trypsin inhibitor.
Add 5 mL of Trypsin-EDTA (0.25% (w/v) Trypsin-0.53 mM EDTA solution, ATCC# 30-2101) solution to flask and incubate for 1 minute, gently tapping the flask observe cells under an inverted microscope until cells detach (usually within 1 to 2 minutes).
Add 6.0 to 8.0 mL of complete growth medium and rinse surface of the flask to detach all cells. Gently pipetting up and down will break cell clumps.
Transfer all cells into a centrifuge bottle or tube and centrifuge at 270 xg for 5 minutes.
Remove and discard the supernatant
Add 10 mL complete growth medium to cell pellet and with 10 mL pipette resuspend the cells gently (create a single-cell suspension).
Add more complete growth medium to cell suspension as needed to plate cells at approximately 5x10⁶/T225 flask.
Place flasks in incubator @ 37°C with a 5% CO₂ in air atmosphere.

Flask/Platexa0

xa0

Cryopreservation：

Growth Area (cm²)xa0

xa0

Culture Conditions：

1xPBS (mL)xa0

xa0

Name of Depositor：

Trypsin/EDTA (mL)xa0

xa0

Year of Origin：

Equalxa0vol. Complete Growth Medium (mL)

xa0

References：

Growth Medium (mL)xa0

xa0

序号	文章标题	操作
1	ATCC细胞运输和处理	查看详情
2	其他海外品牌代理	查看详情
3	ATCC代理	查看详情
4	购买须知	查看详情
5	订购流程	查看详情

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HFF-1

货号: TS209410
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品牌 : TESTOBIO

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产品名称：

HFF-1

商品货号：

TS209410

Organism：

Homo sapiens, human

Tissue：

Skin; foreskin

Cell Type：

Fibroblast

Product Format：

frozen

Morphology：

fibroblast

Culture Properties：

Adherent

Biosafety Level：

Disease：

Normal

Age：

Newborn

Gender：

male

Applications：

Can be used to produce feeder cells

Storage Conditions：

Liquid nitrogen vapor phase

Derivation：

The cell line was established by ATCC in 2003 from normal human foreskin pooled from two individuals.

Clinical Data：

male

Comments：

Complete Growth Medium：

The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).

Subculturing：

Remove and discard culture medium.
Briefly rinse the cell layer with 1X PBS (SCRR-2201) solution to remove all traces of serum, which contain trypsin inhibitor.
Add 5 mL of Trypsin-EDTA (0.25% (w/v) Trypsin-0.53 mM EDTA solution, ATCC# 30-2101) solution to flask and incubate for 1 minute, gently tapping the flask observe cells under an inverted microscope until cells detach (usually within 1 to 2 minutes).
Add 6.0 to 8.0 mL of complete growth medium and rinse surface of the flask to detach all cells. Gently pipetting up and down will break cell clumps.
Transfer all cells into a centrifuge bottle or tube and centrifuge at 270 xg for 5 minutes.
Remove and discard the supernatant
Add 10 mL complete growth medium to cell pellet and with 10 mL pipette resuspend the cells gently (create a single-cell suspension).
Add more complete growth medium to cell suspension as needed to plate cells at approximately 5x10⁶/T225 flask.
Place flasks in incubator @ 37°C with a 5% CO₂ in air atmosphere.