HMEC-1

规格:

货期:

编号:TS209418

品牌:Testobio

产品名称：	HMEC-1
商品货号：	TS209418
Organism：	Homo sapiens, human
Tissue：	dermal endothelium
Cell Type：	dermal microvascular endothelium
Product Format：	frozen
Morphology：	endothelial-like
Culture Properties：	adherent
Biosafety Level：	2 xa0Cells contain SV-40 viral DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	newborn
Gender：	male
Applications：	angiogenesis, leukocyte trafficking, wound healing, inflammation, circulation, tumor growth and metastasis.cancer research, endothelium function, cell trafficking, surface molecule interactions, drug screening, toxicological studies for the pharmaceutical and cosmetic industry
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	Microvascular endothelial cells isolated from human foreskins were transfected with pSVT vector, xa0a pBR-322 based plasmid containing the coding region for Simian virus 40A gene product, large T antigen.
Receptor Expression：	The cells express von Willebrands factor (vWF), cell adhesion molecules ICAM-1 and are capable of acetylated LDL uptake. Ref (Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507)
Comments：	CRL-3243, HMEC-1, has been well characterized and shown to retain many of the characteristics of endothelial cells. These immortalized cells, because they are a continously renewable source of human endothelial microvascular cells, can be used as a replacement for primary human dermal endothelial cells for many research studies.
Complete Growth Medium：	The base medium for this cell line is MCDB131 (without L-Glutamine). To make the complete growth medium, add the following components to the base medium: 10ng/mL Epidermal Growth Factor (EGF) 1 &microg/mL Hydrocortisone 10 mM Glutamine fetal bovine serum (FBS) to a final concentration of 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0 Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.xa0 Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.xa0 Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:6 to 1:12 is recommended. Medium Renewal: Do not feed cells with growth medium between subcultures or cells become very tightly attached and will be difficult to disperse with trypsin-EDTA
Cryopreservation：	Freeze Medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage Temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10, 12 D13S317: 11, 12 D16S539: xa011, 12 D5S818: 12 D7S820: 8, 10 THO1: 7 TPOX: 8, 11 vWA: 15, 17
Name of Depositor：	Kathryn Kellar, Ph. D.
Year of Origin：	November 1990
References：	Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507

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HMEC-1

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产品名称：	HMEC-1
商品货号：	TS209418
Organism：	Homo sapiens, human
Tissue：	dermal endothelium
Cell Type：	dermal microvascular endothelium
Product Format：	frozen
Morphology：	endothelial-like
Culture Properties：	adherent
Biosafety Level：	2 xa0Cells contain SV-40 viral DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	newborn
Gender：	male
Applications：	angiogenesis, leukocyte trafficking, wound healing, inflammation, circulation, tumor growth and metastasis.cancer research, endothelium function, cell trafficking, surface molecule interactions, drug screening, toxicological studies for the pharmaceutical and cosmetic industry
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	Microvascular endothelial cells isolated from human foreskins were transfected with pSVT vector, xa0a pBR-322 based plasmid containing the coding region for Simian virus 40A gene product, large T antigen.
Receptor Expression：	The cells express von Willebrands factor (vWF), cell adhesion molecules ICAM-1 and are capable of acetylated LDL uptake. Ref (Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507)
Comments：	CRL-3243, HMEC-1, has been well characterized and shown to retain many of the characteristics of endothelial cells. These immortalized cells, because they are a continously renewable source of human endothelial microvascular cells, can be used as a replacement for primary human dermal endothelial cells for many research studies.
Complete Growth Medium：	The base medium for this cell line is MCDB131 (without L-Glutamine). To make the complete growth medium, add the following components to the base medium: 10ng/mL Epidermal Growth Factor (EGF) 1 &microg/mL Hydrocortisone 10 mM Glutamine fetal bovine serum (FBS) to a final concentration of 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0 Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.xa0 Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.xa0 Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:6 to 1:12 is recommended. Medium Renewal: Do not feed cells with growth medium between subcultures or cells become very tightly attached and will be difficult to disperse with trypsin-EDTA
Cryopreservation：	Freeze Medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage Temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10, 12 D13S317: 11, 12 D16S539: xa011, 12 D5S818: 12 D7S820: 8, 10 THO1: 7 TPOX: 8, 11 vWA: 15, 17
Name of Depositor：	Kathryn Kellar, Ph. D.
Year of Origin：	November 1990
References：	Ades EW, et al. HMEC-1: Establishment of an Immortalized Human Microvascular Endotheilal Cell Line. J. Invest. Dermatol. 99(6): 683-690, 1992. PubMed: 1361507