Renca

规格:

货期:

编号:TS209423

品牌:Testobio

产品名称：	Renca
商品货号：	TS209423
Organism：	Mus musculus, mouse
Tissue：	kidney
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	renal adenocarcinoma
Age：	6 weeks
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice.
Clinical Data：	male
Tumorigenic：	YES
Comments：	The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver.xa0RefMurphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766xa0RefSalup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425xa0The cells do not express transforming growth factor-beta type II receptor (TbetaR-II) 10414746.xa0RefEngel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746
Complete Growth Medium：	The base medium for this cell line is RPMI-1640 (ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:xa0 10% Fetal Bovine Serum (BSA; ATCC 30-2020) Non-essential amino acids (NEAA; 0.1mM extra) Additional sodium pyruvate (1 mM extra) Additional L-glutamine (2 mM extra) This medium is formulated for use with a 5% CO₂in air atmosphere.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10⁴ to 4 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. We recommend that you subculture when the culture reaches a cell concentration between 8 X 10⁴ and 1.5 X 10⁵ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Every 2 to 3 days.
Cryopreservation：	Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5% Storage temperature liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Population Doubling Time：	approximately 24 hours
Name of Depositor：	R Wiltrout
Year of Origin：	1969
References：	Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766 Salup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425 Engel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746

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Renca

货号: TS209423
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产品名称：	Renca
商品货号：	TS209423
Organism：	Mus musculus, mouse
Tissue：	kidney
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	renal adenocarcinoma
Age：	6 weeks
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice.
Clinical Data：	male
Tumorigenic：	YES
Comments：	The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver.xa0RefMurphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766xa0RefSalup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425xa0The cells do not express transforming growth factor-beta type II receptor (TbetaR-II) 10414746.xa0RefEngel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746
Complete Growth Medium：	The base medium for this cell line is RPMI-1640 (ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:xa0 10% Fetal Bovine Serum (BSA; ATCC 30-2020) Non-essential amino acids (NEAA; 0.1mM extra) Additional sodium pyruvate (1 mM extra) Additional L-glutamine (2 mM extra) This medium is formulated for use with a 5% CO₂in air atmosphere.
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10⁴ to 4 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. We recommend that you subculture when the culture reaches a cell concentration between 8 X 10⁴ and 1.5 X 10⁵ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Every 2 to 3 days.
Cryopreservation：	Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5% Storage temperature liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Population Doubling Time：	approximately 24 hours
Name of Depositor：	R Wiltrout
Year of Origin：	1969
References：	Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766 Salup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425 Engel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746