HCC2935

规格:

货期:

编号:TS209441

品牌:Testobio

产品名称：	HCC2935
商品货号：	TS209441
Organism：	Homo sapiens, human
Tissue：	lung: pleural effusion
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma
Age：	39 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Established from pleural effusion cells of an adenocarcinoma patient who had never smoked.
Clinical Data：	39 years Caucasian male
Comments：	This lung adenocarcinoma has an acquired mutation in the EGFR tyrosine kinase domain (E746 - T751 deletion, S752I).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution or Dulbeccox92s Phosphate Buffered Saline (D-PBS) to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 5 x 10³to 7 x 10³ viable cells/cm² is recommended. Place culture vessels in incubators at 37°C. Maintain cultures at a cell concentration between 8 x 10⁴ and 1 x 10⁵ cells/cm² Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 12 D13S317: 12 D16S539: 12 D5S818: 13 D7S820: 12,13 THO1: 7,9 TPOX: 8 vWA: 17
Population Doubling Time：	105 hours
Name of Depositor：	JD Minna, AF Gazdar
Deposited As：	Homo sapiens
Year of Origin：	May 13, 1999

首页 > 产品中心 > 微生物培养 > 菌株 > null > HCC2935

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

HCC2935

货号: TS209441
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	HCC2935
商品货号：	TS209441
Organism：	Homo sapiens, human
Tissue：	lung: pleural effusion
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	adenocarcinoma
Age：	39 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Established from pleural effusion cells of an adenocarcinoma patient who had never smoked.
Clinical Data：	39 years Caucasian male
Comments：	This lung adenocarcinoma has an acquired mutation in the EGFR tyrosine kinase domain (E746 - T751 deletion, S752I).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution or Dulbeccox92s Phosphate Buffered Saline (D-PBS) to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 5 x 10³to 7 x 10³ viable cells/cm² is recommended. Place culture vessels in incubators at 37°C. Maintain cultures at a cell concentration between 8 x 10⁴ and 1 x 10⁵ cells/cm² Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 12 D13S317: 12 D16S539: 12 D5S818: 13 D7S820: 12,13 THO1: 7,9 TPOX: 8 vWA: 17
Population Doubling Time：	105 hours
Name of Depositor：	JD Minna, AF Gazdar
Deposited As：	Homo sapiens
Year of Origin：	May 13, 1999