| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品货号： | TS210438 |
| Deposited As： | Clostridium difficile (Hall and OToole) Prevot |
| Strain Designations： | VPI 10463 |
| Application： | Enteric Research |
| Isolation： | Abdominal wound |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | Toxinotype: 0 |
| Images： |  |
| Type Strain： | no |
| Comments： | Presence of tcdA and tcdB genes confirmed by PCR. Ref  Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303Toxinotype 0 Binary toxin gene cdtB not amplified by PCR Ribotype 087 Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81). |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Anaerobic |
| Name of Depositor： | TD Wilkins, DM Lyerly |
| Chain of Custody： | ATCC |
| References： | Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 BD GeneOhm Cdiff. BD Becton Dickinson. Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 Kink JA, Williams JA. Antibodies to recombinant Clostridium difficile toxins A and B are an effective treatment and prevent relapse of C. difficile-associated disease in a hamster model of infection. Infect. Immun. 66: 2018-2025, 1998. PubMed: 9573084 |
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| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品货号： | TS210438 |
| Deposited As： | Clostridium difficile (Hall and OToole) Prevot |
| Strain Designations： | VPI 10463 |
| Application： | Enteric Research |
| Isolation： | Abdominal wound |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol®： | no |
| Genotype： | Toxinotype: 0 |
| Images： | |
| Type Strain： | no |
| Comments： | Presence of tcdA and tcdB genes confirmed by PCR. Ref Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303Toxinotype 0 Binary toxin gene cdtB not amplified by PCR Ribotype 087 Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81). |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Anaerobic |
| Name of Depositor： | TD Wilkins, DM Lyerly |
| Chain of Custody： | ATCC |
| References： | Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 BD GeneOhm Cdiff. BD Becton Dickinson. Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 Kink JA, Williams JA. Antibodies to recombinant Clostridium difficile toxins A and B are an effective treatment and prevent relapse of C. difficile-associated disease in a hamster model of infection. Infect. Immun. 66: 2018-2025, 1998. PubMed: 9573084 |
