| 产品名称： | XR-V15B |
|---|---|
| 商品货号： | TS210640 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Derivation： | XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays. |
| Comments： | XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays. These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen. Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. Cells will revert to wild type on treatment with azacytidine. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | G Chu |
| References： | Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631 Errami A, et al. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16: 1519-1526, 1996. PubMed: 8657125 Smider V, et al. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266: 288-291, 1994. PubMed: 7939667 Zdzienicka MZ, et al. Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194: 239-249, 1988. PubMed: 2460764 |
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| 产品名称： | XR-V15B |
|---|---|
| 商品货号： | TS210640 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Derivation： | XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays. |
| Comments： | XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays. These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen. Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. Cells will revert to wild type on treatment with azacytidine. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | G Chu |
| References： | Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631 Errami A, et al. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16: 1519-1526, 1996. PubMed: 8657125 Smider V, et al. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266: 288-291, 1994. PubMed: 7939667 Zdzienicka MZ, et al. Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194: 239-249, 1988. PubMed: 2460764 |
