Y-1

规格:

货期:

编号:TS210647

品牌:Testobio

产品名称：	Y-1
商品货号：	TS210647
Organism：	Mus musculus, mouse
Tissue：	adrenal gland; tumor/cortex
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender：	male
Strain：	LAF1
Applications：	This cell line is useful for the detection of LT toxin (American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.), cholera toxin (U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995), and verotoxin.xa0
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected.
Images：
Clinical Data：	male
Genes Expressed：	steroid hormones
Cellular Products：	steroid hormones
Virus Susceptibility：	Herpes simplex virus Pseudorabies virus Vaccinia virus Vesicular stomatitis virus Human poliovirus 1
Comments：	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: At least 2 times per week (or else hormone production will decrease)
Cryopreservation：	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	G Sato
Deposited As：	Mus musculus
References：	Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699 American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454 Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719 Rainey WE, et al. Adrenocortical cell lines. Mol. Cell Endocrinol. 228(1-2): 23-38, 2004. PubMed: 15541570

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Y-1

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产品名称：	Y-1
商品货号：	TS210647
Organism：	Mus musculus, mouse
Tissue：	adrenal gland; tumor/cortex
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender：	male
Strain：	LAF1
Applications：	This cell line is useful for the detection of LT toxin (American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.), cholera toxin (U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995), and verotoxin.xa0
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected.
Images：
Clinical Data：	male
Genes Expressed：	steroid hormones
Cellular Products：	steroid hormones
Virus Susceptibility：	Herpes simplex virus Pseudorabies virus Vaccinia virus Vesicular stomatitis virus Human poliovirus 1
Comments：	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: At least 2 times per week (or else hormone production will decrease)
Cryopreservation：	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor：	G Sato
Deposited As：	Mus musculus
References：	Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699 American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454 Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719 Rainey WE, et al. Adrenocortical cell lines. Mol. Cell Endocrinol. 228(1-2): 23-38, 2004. PubMed: 15541570