VSW

规格:

货期:

编号:TS210684

品牌:Testobio

产品名称：	VSW
商品货号：	TS210684
Organism：	Daboia russelii, Russells viper
Tissue：	spleen
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor, metastatic
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	model number = 64; range = 33 to 118. Karyotype slightly unstable with stemline number in hypertriploid range. Cells with modal numbers of 28 and 29 macrochromosomes and 34 to 37 microchromosomes. Many cells with a very small chromosome with a median centromere included with macrochromosomes. All cells have 2 to 3 marker chromosomes with terminal centromeres.
Virus Susceptibility：	Vesicular stomatitis virus Rabies virus Human poliovirus 3
Comments：	The cells produce a C type retrovirus.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 30°C. Subcultivation Ratio: 1:2 Medium Renewal: Once a week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 30°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	HF Clark
Deposited As：	Vipera russelli
Year of Origin：	1967
References：	Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059 Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585 Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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VSW

货号: TS210684
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产品名称：	VSW
商品货号：	TS210684
Organism：	Daboia russelii, Russells viper
Tissue：	spleen
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor, metastatic
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	model number = 64; range = 33 to 118. Karyotype slightly unstable with stemline number in hypertriploid range. Cells with modal numbers of 28 and 29 macrochromosomes and 34 to 37 microchromosomes. Many cells with a very small chromosome with a median centromere included with macrochromosomes. All cells have 2 to 3 marker chromosomes with terminal centromeres.
Virus Susceptibility：	Vesicular stomatitis virus Rabies virus Human poliovirus 3
Comments：	The cells produce a C type retrovirus.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 30°C. Subcultivation Ratio: 1:2 Medium Renewal: Once a week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium, 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 30°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	HF Clark
Deposited As：	Vipera russelli
Year of Origin：	1967
References：	Zeigel RF, Clark HF. Electron microscopic observations on a "C"-type virus in cell cultures derived from a tumor-bearing viper. J. Natl. Cancer Inst. 43: 1097-1102, 1969. PubMed: 4982059 Zeigel RF, Clark HF. Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a "C"-type virus-producing cell line. J. Natl. Cancer Inst. 46: 309-321, 1971. PubMed: 5165585 Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.