| 产品名称： | VERO 76 |
|---|---|
| 商品货号： | TS210691 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | adult |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Derivation： | VERO 76 was derived in 1968 by K.M. Johnson and brought to Centers for Disease Control (CDC), Atlanta, GA. It is a derivative of the original VERO. |
| Comments： | When infected with hemorrhagic fever viruses Machupo (Bolivian), Junin (Argentinian), Lassa (African), Marburg or Ebola viruses, these cells exhibited cytopathic effects. Plaques are also produced. VERO 76 has a lower saturation density than the original VERO (ATCC CCL-81). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | EM Earley |
| Deposited As： | Cercopithecus aethiops |
| Year of Origin： | 1968 |
| References： | Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988 Policastro PF, et al. Improved plaque assays for Rickettsia prowazekii in Vero76 cells. J. Clin. Microbiol. 34: 1944-1948, 1996. PubMed: 8818887 Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080 |
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| 产品名称： | VERO 76 |
|---|---|
| 商品货号： | TS210691 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | adult |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Derivation： | VERO 76 was derived in 1968 by K.M. Johnson and brought to Centers for Disease Control (CDC), Atlanta, GA. It is a derivative of the original VERO. |
| Comments： | When infected with hemorrhagic fever viruses Machupo (Bolivian), Junin (Argentinian), Lassa (African), Marburg or Ebola viruses, these cells exhibited cytopathic effects. Plaques are also produced. VERO 76 has a lower saturation density than the original VERO (ATCC CCL-81). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | EM Earley |
| Deposited As： | Cercopithecus aethiops |
| Year of Origin： | 1968 |
| References： | Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988 Policastro PF, et al. Improved plaque assays for Rickettsia prowazekii in Vero76 cells. J. Clin. Microbiol. 34: 1944-1948, 1996. PubMed: 8818887 Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080 |
