| 产品名称： | VH 2 |
|---|---|
| 商品货号： | TS210697 |
| Organism： | Daboia russelii, Russells viper |
| Tissue： | heart |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 35; range = 22 to 67. Karyotype stable with a near-diploid stemline number. The cells have 16 macrochromosomes and from 17 to 20 microchromosomes. |
| Virus Susceptibility： | Herpes simplex virus
Vaccinia virus Vesicular stomatitis virus Human poliovirus 1 |
| Comments： | The genus name has been reclassified to Daboia russelii formerly known as Vipera russelii |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
xa0 Subcultivation Ratio: 1:2 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 30°C |
| Name of Depositor： | HF Clark |
| Deposited As： | Vipera russelli |
| Year of Origin： | June, 1968 |
| References： | . . In Vitro 6: 376, 1971. Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | VH 2 |
|---|---|
| 商品货号： | TS210697 |
| Organism： | Daboia russelii, Russells viper |
| Tissue： | heart |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 35; range = 22 to 67. Karyotype stable with a near-diploid stemline number. The cells have 16 macrochromosomes and from 17 to 20 microchromosomes. |
| Virus Susceptibility： | Herpes simplex virus
Vaccinia virus Vesicular stomatitis virus Human poliovirus 1 |
| Comments： | The genus name has been reclassified to Daboia russelii formerly known as Vipera russelii |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
xa0 Subcultivation Ratio: 1:2 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 30°C |
| Name of Depositor： | HF Clark |
| Deposited As： | Vipera russelli |
| Year of Origin： | June, 1968 |
| References： | . . In Vitro 6: 376, 1971. Clark HF, et al. Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli. J. Natl. Cancer Inst. 51: 645-654, 1973. PubMed: 4358139 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
