| 产品名称： | UCD/AB 6.01 |
|---|---|
| 商品货号： | TS210717 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Cancer |
| Applications： | The antibody is specific for type 8 cytokeratin (52000 dalton). It cross-reacts somewhat with types 18 and 19 cytokeratins (46000 dalton and 40000 dalton, respectively). The antibody can be used to detect cytokeratin released by neoplastic cells into serum. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a human mammary carcinoma cell line (MCF7, see ATCC HTB-22) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a human mammary carcinoma cell line (MCF7, see ATCC HTB-22) |
| Comments： | Animals were immunized with the MCF7 human mammary cancer cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for type 8 cytokeratin (52000 dalton). It cross-reacts somewhat with types 18 and 19 cytokeratins (46000 dalton and 40000 dalton, respectively). The antibody can be used to detect cytokeratin released by neoplastic cells into serum. |
| Complete Growth Medium： | Culture medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.15 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Reference: U.S. Pat. 4,775,620. Deposited on behalf of:xa0 University of California xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Davis, CA Cell lines and hybridomas deposited for patent purposes are not always screened for contamination, antibody production or characterized by the ATCC. Release of a culture, the use of which may be claimed in a patent, from the ATCC during the effective term of any such patent is not meant to carry with it, and does not grant any license, express or implied, under any patent, or the right to use a culture in any process described in a patent. The above culture was deposited in the ATCC in connection with a patent application. Copies of U.S. Patents may be obtained from the Commissioner of Patents, U.S. Patent and Trademark Office, Box 9, Washington, D.C. 20231. A coupon is required which is purchased through the office above, and on which the information is submitted to request the patent. xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 (1/98) .CCLSFTY.FACTSHTS.VXL |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium, 90%; DMSO, 10% |
| Name of Depositor： | Regents of the University of California |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. US Patent 4,775,620 dated Oct 4 1988 |
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| 产品名称： | UCD/AB 6.01 |
|---|---|
| 商品货号： | TS210717 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Cancer |
| Applications： | The antibody is specific for type 8 cytokeratin (52000 dalton). It cross-reacts somewhat with types 18 and 19 cytokeratins (46000 dalton and 40000 dalton, respectively). The antibody can be used to detect cytokeratin released by neoplastic cells into serum. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a human mammary carcinoma cell line (MCF7, see ATCC HTB-22) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a human mammary carcinoma cell line (MCF7, see ATCC HTB-22) |
| Comments： | Animals were immunized with the MCF7 human mammary cancer cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for type 8 cytokeratin (52000 dalton). It cross-reacts somewhat with types 18 and 19 cytokeratins (46000 dalton and 40000 dalton, respectively). The antibody can be used to detect cytokeratin released by neoplastic cells into serum. |
| Complete Growth Medium： | Culture medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.15 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%. Reference: U.S. Pat. 4,775,620. Deposited on behalf of:xa0 University of California xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Davis, CA Cell lines and hybridomas deposited for patent purposes are not always screened for contamination, antibody production or characterized by the ATCC. Release of a culture, the use of which may be claimed in a patent, from the ATCC during the effective term of any such patent is not meant to carry with it, and does not grant any license, express or implied, under any patent, or the right to use a culture in any process described in a patent. The above culture was deposited in the ATCC in connection with a patent application. Copies of U.S. Patents may be obtained from the Commissioner of Patents, U.S. Patent and Trademark Office, Box 9, Washington, D.C. 20231. A coupon is required which is purchased through the office above, and on which the information is submitted to request the patent. xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 (1/98) .CCLSFTY.FACTSHTS.VXL |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium, 90%; DMSO, 10% |
| Name of Depositor： | Regents of the University of California |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. US Patent 4,775,620 dated Oct 4 1988 |
