TOV-21G

规格:

货期:

编号:TS210745

品牌:Testobio

产品名称：	TOV-21G
商品货号：	TS210745
Organism：	Homo sapiens, human
Tissue：	ovary
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	grade 3, stage III,primary malignant adenocarcinoma; clear cell carcinoma
Age：	62 years
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype：	47, XX, +10 RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Images：
Derivation：	This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer.
Clinical Data：	female
Oncogene：	p53 + (wild type)
Genes Expressed：	keratin
Cellular Products：	keratin
Tumorigenic：	Yes
Effects：	Yes, form colonies in soft agar Yes, the cells are tumorigenic in nude mice
Comments：	Like OV-90 (ATCC CRL-11732), the OV-21G (TS210745) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731) cell line does not exhibit a deletion at chromosome 3p24.
Complete Growth Medium：	The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 3 to 4 days
Cryopreservation：	Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: Air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 13,15 D13S317: 11,12 D16S539: 10,12 D5S818: 12,13 D7S820: 12 THO1: 7,9.3 TPOX: 8,11 vWA: 17
Name of Depositor：	University of Montreal
Deposited As：	human
U.S. Patent Number：	5,710,038
Year of Origin：	1991
References：	Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998 Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

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TOV-21G

货号: TS210745
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产品名称：	TOV-21G
商品货号：	TS210745
Organism：	Homo sapiens, human
Tissue：	ovary
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	grade 3, stage III,primary malignant adenocarcinoma; clear cell carcinoma
Age：	62 years
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype：	47, XX, +10 RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Images：
Derivation：	This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer.
Clinical Data：	female
Oncogene：	p53 + (wild type)
Genes Expressed：	keratin
Cellular Products：	keratin
Tumorigenic：	Yes
Effects：	Yes, form colonies in soft agar Yes, the cells are tumorigenic in nude mice
Comments：	Like OV-90 (ATCC CRL-11732), the OV-21G (TS210745) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731) cell line does not exhibit a deletion at chromosome 3p24.
Complete Growth Medium：	The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 3 to 4 days
Cryopreservation：	Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: Air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 13,15 D13S317: 11,12 D16S539: 10,12 D5S818: 12,13 D7S820: 12 THO1: 7,9.3 TPOX: 8,11 vWA: 17
Name of Depositor：	University of Montreal
Deposited As：	human
U.S. Patent Number：	5,710,038
Year of Origin：	1991
References：	Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998 Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993