| 产品名称： | TRA-171 |
|---|---|
| 商品货号： | TS210747 |
| Organism： | Toxorhynchites amboinensis, mosquito |
| Tissue： | larva, whole |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | larva |
| Storage Conditions： | liquid nitrogen vapor phase |
| Complete Growth Medium： | MThe base medium for this cell line is a 1:1 mixture of MM (Mitsuhashi and Maramorosch) and VP12 Medium (J. Med. Entomol. 6:432-439, 1969. PubMed: 5360492)
To make the complete growth medium, add the following components to the base medium:
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published byxa0Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 28°C
Atmosphere: Air, 100% |
| Population Doubling Time： | 28 hrs |
| Name of Depositor： | G Kuno |
| Deposited As： | Toxorhynchites amboinensis |
| References： | Kuno G. New cell line. A continuous cell line of a nonhematophagous mosquito, Toxorhynchites amboinensis. In Vitro 16: 915-917, 1980. Varma MG, Pudney M. The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432-439, 1969. PubMed: 5360492 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | TRA-171 |
|---|---|
| 商品货号： | TS210747 |
| Organism： | Toxorhynchites amboinensis, mosquito |
| Tissue： | larva, whole |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | larva |
| Storage Conditions： | liquid nitrogen vapor phase |
| Complete Growth Medium： | MThe base medium for this cell line is a 1:1 mixture of MM (Mitsuhashi and Maramorosch) and VP12 Medium (J. Med. Entomol. 6:432-439, 1969. PubMed: 5360492)
To make the complete growth medium, add the following components to the base medium:
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published byxa0Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 28°C
Atmosphere: Air, 100% |
| Population Doubling Time： | 28 hrs |
| Name of Depositor： | G Kuno |
| Deposited As： | Toxorhynchites amboinensis |
| References： | Kuno G. New cell line. A continuous cell line of a nonhematophagous mosquito, Toxorhynchites amboinensis. In Vitro 16: 915-917, 1980. Varma MG, Pudney M. The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432-439, 1969. PubMed: 5360492 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
