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Te39
Te39
规格:
货期:
编号:TS210772
品牌:Testobio
产品名称: Te39
商品货号: TS210772
Organism: human (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: Cancer
Strain:

Strain:xa0 BALB/c (myeloma)

Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation:
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Clinical Data:
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Genes Expressed:
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Cellular Products:
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Comments:
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Complete Growth Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Isotype: IgM
Name of Depositor: Sloan-Kettering Institute for Cancer Research
Deposited As: human (B cell); mouse (myeloma)
U.S. Patent Number:
References:

Thomson TM, et al. Human monoclonal antibodies to cell surface antigens. US Patent 4,695,538 dated Sep 22 1987

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Te39

  • 货号: TS210772
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  • 品牌 : TESTOBIO
产品名称: Te39
商品货号: TS210772
Organism: human (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: Cancer
Strain:

Strain:xa0 BALB/c (myeloma)

Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation:
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Clinical Data:
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Genes Expressed:
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Cellular Products:
immunoglobulin; monoclonal antibody; against an intracellular antigen expressed in human malignant cells (cancer, tumor)
Comments:
Lymphocytes were obtained from the regional lymph nodes of a patient who had colon cancer.
Lymphocytes were fused with NS-1 myeloma cells.
Complete Growth Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation:
culture medium 95%; DMSO, 5%
Culture Conditions:
Temperature: 37.0°C
Isotype: IgM
Name of Depositor: Sloan-Kettering Institute for Cancer Research
Deposited As: human (B cell); mouse (myeloma)
U.S. Patent Number:
References:

Thomson TM, et al. Human monoclonal antibodies to cell surface antigens. US Patent 4,695,538 dated Sep 22 1987

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