TCCSUP

规格:

货期:

编号:TS210779

品牌:Testobio

产品名称：	TCCSUP
商品货号：	TS210779
Organism：	Homo sapiens, human
Tissue：	urinary bladder
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	grade IV transitional cell carcinoma
Age：	67 years
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	(P12 and 35) hypotetraploid with marker chromosomes
Derivation：	The TCCSUP line was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) in the neck of the urinary bladder.
Clinical Data：	female
Antigen Expression：	HLA 2, 3, 7, 12
Comments：	The patient had a 4 month history of hematuria prior to removal of the tumor. Metastases to the bone marrow were discovered later. Studies on ultrastructure indicated presence of microvilli and lipid bodies but no desmosomes were observed.
Complete Growth Medium：	Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids (ATCC 30-2003) and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommendedxa0 Medium Renewal: 2 to 3 times per week Protocol: Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 11,14 D16S539: 9,11 D5S818: 12 D7S820: 8,9 THO1: 6,9.3 TPOX: 8 vWA: 14,16
Isoenzymes：	AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 2 PGM3, 1
Name of Depositor：	C OToole
References：	OToole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: OToole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125. Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756

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TCCSUP

货号: TS210779
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产品名称：	TCCSUP
商品货号：	TS210779
Organism：	Homo sapiens, human
Tissue：	urinary bladder
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	grade IV transitional cell carcinoma
Age：	67 years
Gender：	female
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	(P12 and 35) hypotetraploid with marker chromosomes
Derivation：	The TCCSUP line was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) in the neck of the urinary bladder.
Clinical Data：	female
Antigen Expression：	HLA 2, 3, 7, 12
Comments：	The patient had a 4 month history of hematuria prior to removal of the tumor. Metastases to the bone marrow were discovered later. Studies on ultrastructure indicated presence of microvilli and lipid bodies but no desmosomes were observed.
Complete Growth Medium：	Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids (ATCC 30-2003) and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subculture every 6 to 8 days. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommendedxa0 Medium Renewal: 2 to 3 times per week Protocol: Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10 D13S317: 11,14 D16S539: 9,11 D5S818: 12 D7S820: 8,9 THO1: 6,9.3 TPOX: 8 vWA: 14,16
Isoenzymes：	AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 2 PGM3, 1
Name of Depositor：	C OToole
References：	OToole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: OToole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125. Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 Nayak SK, et al. A cell line from an anaplastic transitional cell carcinoma of human urinary bladder. Br. J. Cancer 35: 142-151, 1977. PubMed: 836756