Tb 1 Lu

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货期:

编号:TS210791

品牌:Testobio

产品名称：	Tb 1 Lu
商品货号：	TS210791
Organism：	Tadarida brasiliensis, bat, free-tailed
Tissue：	lung
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Gender：	female
Storage Conditions：	liquid nitrogen vapor pahse
Karyotype：	modal number = 48; range = 42 to 53 Polyploidy about 1%. Dicentric chromosomes or chromosomes with chromatids fused at distal end in 6% of cells.
Virus Resistance：	adenovirus 5; coxsackievirus A9, B5; herpes simplex; bovine diarrhea; infectious bovine rhinotracheitis; poliovirus 2
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Twice per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. IanFreshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	AJ Kniazeff, D Constantine, WA Nelson-Rees, D Schmidt
Deposited As：	Tadarida brasiliensis
Year of Origin：	October, 1965
References：	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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Tb 1 Lu

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产品名称：	Tb 1 Lu
商品货号：	TS210791
Organism：	Tadarida brasiliensis, bat, free-tailed
Tissue：	lung
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Gender：	female
Storage Conditions：	liquid nitrogen vapor pahse
Karyotype：	modal number = 48; range = 42 to 53 Polyploidy about 1%. Dicentric chromosomes or chromosomes with chromatids fused at distal end in 6% of cells.
Virus Resistance：	adenovirus 5; coxsackievirus A9, B5; herpes simplex; bovine diarrhea; infectious bovine rhinotracheitis; poliovirus 2
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Twice per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. IanFreshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	AJ Kniazeff, D Constantine, WA Nelson-Rees, D Schmidt
Deposited As：	Tadarida brasiliensis
Year of Origin：	October, 1965
References：	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.