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SW48 [SW-48]
SW48 [SW-48]
规格:
货期:
编号:TS210818
品牌:Testobio
产品名称： SW48 SW-48
商品货号： TS210818
Organism： Homo sapiens, human
Tissue： colon
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： Dukes type C, grade IV, colorectal adenocarcinoma
Age： 82 years
Gender： female
Ethnicity： Caucasian
Applications：
This cell line is a suitable transfection host.
Storage Conditions： liquid nitrogen vapor phase
Images：
Clinical Data：
female
82 years
Caucasian
Antigen Expression： HLA A32, A33, B27, B35; blood type AB; Rh+
Genes Expressed：
carcinoembryonic antigen (CEA) 0.6 ng/106 cells/10 days; keratin
Cellular Products：
carcinoembryonic antigen (CEA) 0.6 ng/10 exp6 cells/10 days; keratin
Tumorigenic： Yes
Effects：
Yes, in nude mice
(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments：
CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing：
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Culture never becomes 100% confluent. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. .
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 100%
Temperature: 37°C
STR Profile：
Amelogenin: X
CSF1PO: 9, 10
D13S317: 11, 12
D16S539: 11, 13
D5S818: 10, 14
D7S820: 9, 10
THO1: 6, 9.3
TPOX: 8
vWA: 18, 20, 21
Isoenzymes：
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Name of Depositor： A Leibovitz
Deposited As： Homo sapiens
References：

Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

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  • 周雪
    13372109301

SW48 [SW-48]

  • 货号: TS210818
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： SW48 SW-48
商品货号： TS210818
Organism： Homo sapiens, human
Tissue： colon
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： Dukes type C, grade IV, colorectal adenocarcinoma
Age： 82 years
Gender： female
Ethnicity： Caucasian
Applications：
This cell line is a suitable transfection host.
Storage Conditions： liquid nitrogen vapor phase
Images：
Clinical Data：
female
82 years
Caucasian
Antigen Expression： HLA A32, A33, B27, B35; blood type AB; Rh+
Genes Expressed：
carcinoembryonic antigen (CEA) 0.6 ng/106 cells/10 days; keratin
Cellular Products：
carcinoembryonic antigen (CEA) 0.6 ng/10 exp6 cells/10 days; keratin
Tumorigenic： Yes
Effects：
Yes, in nude mice
(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments：
CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing：
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Culture never becomes 100% confluent. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. .
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation：
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Atmosphere: air, 100%
Temperature: 37°C
STR Profile：
Amelogenin: X
CSF1PO: 9, 10
D13S317: 11, 12
D16S539: 11, 13
D5S818: 10, 14
D7S820: 9, 10
THO1: 6, 9.3
TPOX: 8
vWA: 18, 20, 21
Isoenzymes：
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
Name of Depositor： A Leibovitz
Deposited As： Homo sapiens
References：

Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501

Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

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