| 产品名称： | SW1463 SW 1463, SW-1463 |
|---|---|
| 商品货号： | TS210826 |
| Organism： | Homo sapiens, human |
| Tissue： | rectum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C, colorectal adenocarcinoma |
| Age： | 66 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was derived from a grade II mucous producing and grade III solid tumor. |
| Clinical Data： | 66 years Caucasian female |
| Antigen Expression： | blood type A; Rh + |
| Genes Expressed： | carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin |
| Cellular Products： | carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Subculture just prior to confluency
Medium Renewal: 1 to 2 times per week
|
| Culture Conditions： | Atmosphere: Air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 12,13 D16S539: 11 D5S818: 13,14 D7S820: 9 THO1: 6,7 TPOX: 8,11 vWA: 16 |
| Isoenzymes： | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| References： | Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SW1463 SW 1463, SW-1463 |
|---|---|
| 商品货号： | TS210826 |
| Organism： | Homo sapiens, human |
| Tissue： | rectum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C, colorectal adenocarcinoma |
| Age： | 66 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was derived from a grade II mucous producing and grade III solid tumor. |
| Clinical Data： | 66 years Caucasian female |
| Antigen Expression： | blood type A; Rh + |
| Genes Expressed： | carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin |
| Cellular Products： | carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Subculture just prior to confluency
Medium Renewal: 1 to 2 times per week
|
| Culture Conditions： | Atmosphere: Air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 12,13 D16S539: 11 D5S818: 13,14 D7S820: 9 THO1: 6,7 TPOX: 8,11 vWA: 16 |
| Isoenzymes： | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | A Leibovitz |
| Deposited As： | Homo sapiens |
| References： | Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 |
