SW403 [SW-403]

规格:

货期:

编号:TS210827

品牌:Testobio

产品名称：	SW403 SW-403
商品货号：	TS210827
Organism：	Homo sapiens, human
Tissue：	colon
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Dukes type C, grade III, colorectal adenocarcinoma
Age：	51 years
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 68; range = 59 to 73. The stemline chromosome number is near triploid with 2S component occurring at about 1.4% and 10 marker chromosomes were common to S metaphases. Except for monosomic 1, 13 and 22, and pentasomic 20, most normal chromosome types ranged from disomy to tetrasomy. The cell line is karyotypically very homogeneous and stable.
Clinical Data：	51 years Caucasian female
Antigen Expression：	blood type O
Genes Expressed：	carcinoembryonic antigen (CEA) 155 ng/10⁶ cells/10 days; keratin,
Cellular Products：	carcinoembryonic antigen (CEA) 155 ng/10 exp6 cells/10 days; keratin
Tumorigenic：	Yes
Effects：	Yes, in nude mice. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells.
Comments：	CSAp negative (CSAp-). Colon antigen 3, positive. The cells are positive for keratin by immunoperoxidase staining. xa0
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C without CO₂. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Two to three times weekly
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 100% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10,13 D13S317: 13 D16S539: 10,12 D5S818: 11 D7S820: 8,9 THO1: 6 TPOX: 8,9 vWA: 14,18
Isoenzymes：	ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2
Name of Depositor：	A Leibovitz
Deposited As：	Homo sapiens
References：	Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

首页 > 产品中心 > 微生物培养 > 菌株 > null > SW403 [SW-403]

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

SW403 [SW-403]

货号: TS210827
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	SW403 SW-403
商品货号：	TS210827
Organism：	Homo sapiens, human
Tissue：	colon
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Dukes type C, grade III, colorectal adenocarcinoma
Age：	51 years
Gender：	female
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 68; range = 59 to 73. The stemline chromosome number is near triploid with 2S component occurring at about 1.4% and 10 marker chromosomes were common to S metaphases. Except for monosomic 1, 13 and 22, and pentasomic 20, most normal chromosome types ranged from disomy to tetrasomy. The cell line is karyotypically very homogeneous and stable.
Clinical Data：	51 years Caucasian female
Antigen Expression：	blood type O
Genes Expressed：	carcinoembryonic antigen (CEA) 155 ng/10⁶ cells/10 days; keratin,
Cellular Products：	carcinoembryonic antigen (CEA) 155 ng/10 exp6 cells/10 days; keratin
Tumorigenic：	Yes
Effects：	Yes, in nude mice. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells.
Comments：	CSAp negative (CSAp-). Colon antigen 3, positive. The cells are positive for keratin by immunoperoxidase staining. xa0
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C without CO₂. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Two to three times weekly
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 100% Temperature: 37°C
STR Profile：	Amelogenin: X CSF1PO: 10,13 D13S317: 13 D16S539: 10,12 D5S818: 11 D7S820: 8,9 THO1: 6 TPOX: 8,9 vWA: 14,18
Isoenzymes：	ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2
Name of Depositor：	A Leibovitz
Deposited As：	Homo sapiens
References：	Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066