SW 1573 [SW-1573, SW1573]
SW 1573 [SW-1573, SW1573]
规格:
货期:
编号:TS210828
品牌:Testobio
| 产品名称： | SW 1573 SW-1573, SW1573 |
|---|---|
| 商品货号： | TS210828 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | alveolar cell carcinoma |
| Age： | 44 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Derivation： | The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma. |
| Clinical Data： | female 44 years Caucasian |
| Antigen Expression： | Blood Type O; Rh + |
| Comments： | The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:10 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 12,14 D5S818: 12,13 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 20 |
| Name of Depositor： | W McCombs |
| Deposited As： | Homo sapiens |
| References： | Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474 |
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SW 1573 [SW-1573, SW1573]
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| 产品名称： | SW 1573 SW-1573, SW1573 |
|---|---|
| 商品货号： | TS210828 |
| Organism： | Homo sapiens, human |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | alveolar cell carcinoma |
| Age： | 44 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Derivation： | The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma. |
| Clinical Data： | female 44 years Caucasian |
| Antigen Expression： | Blood Type O; Rh + |
| Comments： | The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:10 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 12,14 D5S818: 12,13 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 20 |
| Name of Depositor： | W McCombs |
| Deposited As： | Homo sapiens |
| References： | Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474 |
