| 产品名称： | SW 1990 SW-1990, SW1990 |
|---|---|
| 商品货号： | TS210835 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas; Derived from metastatic site:xa0spleen |
| Product Format： | frozen |
| Morphology： | Epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 56 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | near triploid; range = 67 to 75 |
| Images： |  |
| Derivation： | The SW 1990 line was established in 1978 from a spleen metastasis of a grade II pancreatic adenocarcinoma derived from the exocrine pancreas.
|
| Clinical Data： | 56 years Caucasian male |
| Antigen Expression： | Blood Type A; Rh + |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The cells have a reported plating efficiency of 29%. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： |
Subcultivation Ratio:xa0A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal:xa02 to 3 times per week
|
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 100% |
| STR Profile： | D5S818: 12, 13 D13S317: 8, 12 D7S820: 9, 10 D16S539: 13 vWA: 17 THO1: 9.3 Amelogenin: X TPOX: 8, 9 CSF1PO: 10, 12 |
| Population Doubling Time： | 64 hrs |
| Name of Depositor： | W McCombs |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1978 |
| References： | Kyriazis AP, et al. Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse. Cancer Res. 43: 4393-4401, 1983. PubMed: 6871872 |
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| 产品名称： | SW 1990 SW-1990, SW1990 |
|---|---|
| 商品货号： | TS210835 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas; Derived from metastatic site:xa0spleen |
| Product Format： | frozen |
| Morphology： | Epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 56 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | near triploid; range = 67 to 75 |
| Images： | |
| Derivation： | The SW 1990 line was established in 1978 from a spleen metastasis of a grade II pancreatic adenocarcinoma derived from the exocrine pancreas.
|
| Clinical Data： | 56 years Caucasian male |
| Antigen Expression： | Blood Type A; Rh + |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The cells have a reported plating efficiency of 29%. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： |
Subcultivation Ratio:xa0A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal:xa02 to 3 times per week
|
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 100% |
| STR Profile： | D5S818: 12, 13 D13S317: 8, 12 D7S820: 9, 10 D16S539: 13 vWA: 17 THO1: 9.3 Amelogenin: X TPOX: 8, 9 CSF1PO: 10, 12 |
| Population Doubling Time： | 64 hrs |
| Name of Depositor： | W McCombs |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1978 |
| References： | Kyriazis AP, et al. Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse. Cancer Res. 43: 4393-4401, 1983. PubMed: 6871872 |
