SVEC4-10

规格:

货期:

编号:TS210852

品牌:Testobio

产品名称：	SVEC4-10
商品货号：	TS210852
Organism：	Mus musculus, mouse
Tissue：	axillary lymph node/vascular epithelium
Cell Type：	endothelial, SV40 transformed
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Cells contain polyomavirus DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Gender：	male
Strain：	C3H/HeJ
Derivation：	SVEC4-10 is an endothelial cell line derived by SV40 (strain 4A) transformation of endothelial cells from axillary lymph node vessels.
Clinical Data：	male
Antigen Expression：	H-2 K; Factor VIII related antigen; VCAM
Receptor Expression：	high affinity receptors for low density lipoprotein (LDL)
Tumorigenic：	Yes
Effects：	Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks
Comments：	They grow indefinitely without special additives and are well differentiated, responding like normal endothelial cells to some interleukins and to extracellular matrix signals for tube-like differentiation. When grown on a synthetic basement-like membrane, SVEC4-10 forms branching tube-like networks. They specifically bind mouse lymphocytes in vitro. The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, and are susceptible to lysis by anti SV40 H-2 k CTL clones. Interferon gamma (IFN-gamma) induces expression of MHC class II antigen in a time course identical to that of normal endothelial cells. The cells express vascular cell adhesion molecule (VCAM), and stain positively for SV40 T antigen. Tumor necrosis factor alpha (TNF alpha) induces SVEC4-10 to undergo reversible transition to a spindle shaped morphology. SVEC4-10 is the parental line for a series of endothelial cell clones (ATCC CRL-2160, ATCC CRL-2161, ATCC CRL-2162, ATCC CRL-2163, ATCC CRL-2167, ATCC CRL-2168 and ATCC CRL-2171).
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mMxa0EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C Subculture Ratio: 1:4 to 1:6 Medium Renewal: 2 to 3 times a week. Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Population Doubling Time：	24 to 30 hrs
Name of Depositor：	KA OConnell
References：	OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299

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SVEC4-10

货号: TS210852
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产品名称：	SVEC4-10
商品货号：	TS210852
Organism：	Mus musculus, mouse
Tissue：	axillary lymph node/vascular epithelium
Cell Type：	endothelial, SV40 transformed
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Cells contain polyomavirus DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Gender：	male
Strain：	C3H/HeJ
Derivation：	SVEC4-10 is an endothelial cell line derived by SV40 (strain 4A) transformation of endothelial cells from axillary lymph node vessels.
Clinical Data：	male
Antigen Expression：	H-2 K; Factor VIII related antigen; VCAM
Receptor Expression：	high affinity receptors for low density lipoprotein (LDL)
Tumorigenic：	Yes
Effects：	Yes, the cells induce spindle tumors with some of the histopathologic characteristics of human Kaposi Sarcoma after a latency period of approximately 14 weeks
Comments：	They grow indefinitely without special additives and are well differentiated, responding like normal endothelial cells to some interleukins and to extracellular matrix signals for tube-like differentiation. When grown on a synthetic basement-like membrane, SVEC4-10 forms branching tube-like networks. They specifically bind mouse lymphocytes in vitro. The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, and are susceptible to lysis by anti SV40 H-2 k CTL clones. Interferon gamma (IFN-gamma) induces expression of MHC class II antigen in a time course identical to that of normal endothelial cells. The cells express vascular cell adhesion molecule (VCAM), and stain positively for SV40 T antigen. Tumor necrosis factor alpha (TNF alpha) induces SVEC4-10 to undergo reversible transition to a spindle shaped morphology. SVEC4-10 is the parental line for a series of endothelial cell clones (ATCC CRL-2160, ATCC CRL-2161, ATCC CRL-2162, ATCC CRL-2163, ATCC CRL-2167, ATCC CRL-2168 and ATCC CRL-2171).
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mMxa0EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C Subculture Ratio: 1:4 to 1:6 Medium Renewal: 2 to 3 times a week. Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Population Doubling Time：	24 to 30 hrs
Name of Depositor：	KA OConnell
References：	OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299