SR-4987

规格:

货期:

编号:TS210858

品牌:Testobio

产品名称：	SR-4987
商品货号：	TS210858
Organism：	Mus musculus, mouse
Tissue：	bone marrow
Cell Type：	virus transformed
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Leukemia
Age：	5 months
Gender：	female
Strain：	BDF1
Applications：	transfection host
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	tetraploid
Derivation：	The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
Clinical Data：	female
Genes Expressed：	macrophage colony stimulating factor (MCSF)
Cellular Products：	macrophage colony stimulating factor (MCSF)
Tumorigenic：	Yes
Effects：	Yes, produce sarcomas in syngeneic mice
Comments：	The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV). The cells produce MCSF, and are highly sensitive to fibroblast growth factor (FGF). They are poorly clonogenic in soft agar. Neither interleukin 3 (IL-3) or granulocyte colony stimulating factor (GCSF) were detected in SR-4987 conditioned medium.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:8 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Population Doubling Time：	15 hrs
Name of Depositor：	G Zaleskis
Deposited As：	Mus musculus
References：	Pessina A, et al. Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology 8: 93-102, 1992. PubMed: 1382506 Pessina A, et al. Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25: 536-541, 1997. PubMed: 9197333 Pessina A, et al. Role of SR-4987 stromal cells in the modulation of doxorubicin toxicity to in vitro granulocyte-macrophage progenitors (CFU-GM). Life Sci. 65: 513-523, 1999. PubMed: 10462078 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

首页 > 产品中心 > 微生物培养 > 菌株 > null > SR-4987

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

SR-4987

货号: TS210858
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	SR-4987
商品货号：	TS210858
Organism：	Mus musculus, mouse
Tissue：	bone marrow
Cell Type：	virus transformed
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Leukemia
Age：	5 months
Gender：	female
Strain：	BDF1
Applications：	transfection host
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	tetraploid
Derivation：	The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
Clinical Data：	female
Genes Expressed：	macrophage colony stimulating factor (MCSF)
Cellular Products：	macrophage colony stimulating factor (MCSF)
Tumorigenic：	Yes
Effects：	Yes, produce sarcomas in syngeneic mice
Comments：	The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV). The cells produce MCSF, and are highly sensitive to fibroblast growth factor (FGF). They are poorly clonogenic in soft agar. Neither interleukin 3 (IL-3) or granulocyte colony stimulating factor (GCSF) were detected in SR-4987 conditioned medium.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:8 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Population Doubling Time：	15 hrs
Name of Depositor：	G Zaleskis
Deposited As：	Mus musculus
References：	Pessina A, et al. Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology 8: 93-102, 1992. PubMed: 1382506 Pessina A, et al. Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25: 536-541, 1997. PubMed: 9197333 Pessina A, et al. Role of SR-4987 stromal cells in the modulation of doxorubicin toxicity to in vitro granulocyte-macrophage progenitors (CFU-GM). Life Sci. 65: 513-523, 1999. PubMed: 10462078 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.