| 产品名称： | ST486 |
|---|---|
| 商品货号： | TS210861 |
| Organism： | Homo sapiens, human |
| Tissue： | ascites |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Antigen Expression： | HLA A2, A10 (Aw25), B5 (Bw52), B18 |
| Receptor Expression： | complement |
| Genes Expressed： | IgM; kappa light chain, immunoglobulin (surface and secreted) |
| Cellular Products： | IgM; kappa light chain immunoglobulin (surface and secreted) |
| Comments： | The cells are EBNA negative and are negative for Fc receptors. Approximately 2% of the cells express commplement receptors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 8,12 D16S539: 9,12 D5S818: 11,12 D7S820: 7,12 THO1: 7,9 TPOX: 8,11 vWA: 17,18 |
| Population Doubling Time： | 19 to 20 hrs |
| Name of Depositor： | IT Magrath |
| Deposited As： | Homo sapiens |
| References： | Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722 |
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| 产品名称： | ST486 |
|---|---|
| 商品货号： | TS210861 |
| Organism： | Homo sapiens, human |
| Tissue： | ascites |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Burkitts lymphoma |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Antigen Expression： | HLA A2, A10 (Aw25), B5 (Bw52), B18 |
| Receptor Expression： | complement |
| Genes Expressed： | IgM; kappa light chain, immunoglobulin (surface and secreted) |
| Cellular Products： | IgM; kappa light chain immunoglobulin (surface and secreted) |
| Comments： | The cells are EBNA negative and are negative for Fc receptors. Approximately 2% of the cells express commplement receptors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 8,12 D16S539: 9,12 D5S818: 11,12 D7S820: 7,12 THO1: 7,9 TPOX: 8,11 vWA: 17,18 |
| Population Doubling Time： | 19 to 20 hrs |
| Name of Depositor： | IT Magrath |
| Deposited As： | Homo sapiens |
| References： | Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722 |
