Src++

规格:

货期:

编号:TS210862

品牌:Testobio

产品名称：	Src++
商品货号：	TS210862
Organism：	Mus musculus, mouse
Tissue：	embryo
Cell Type：	immortalized with SV40 large T antigen
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Papovavirus; SV40 viral sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	9.5 days gestation
Applications：	The cells may be used as littermate controls for experiments involving SYF cells.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The Src++ cell line was derived from a control mouse embryo. The cells were immortalized by infection with a retroviral vector transducing the SV40 large T antigen. xa0
Comments：	The Src++ cell line expresses endogenous wild type tyrosine kinase Src, but lacks expression of the tyrosine kinases Yes and Fyn.xa0 Cells deficient for Src, Yes, and Fyn are available as SYF (ATCC CRL-2459).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C
Name of Depositor：	RA Klinghoffer, P Soriano
Deposited As：	mouse
References：	Klinghoffer RA, et al. Src family kinases are required for integrin but not PDGFR signal transduction. EMBO J. 18: 2459-2471, 1999. PubMed: 10228160 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

首页 > 产品中心 > 微生物培养 > 菌株 > null > Src++

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

Src++

货号: TS210862
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	Src++
商品货号：	TS210862
Organism：	Mus musculus, mouse
Tissue：	embryo
Cell Type：	immortalized with SV40 large T antigen
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Papovavirus; SV40 viral sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	9.5 days gestation
Applications：	The cells may be used as littermate controls for experiments involving SYF cells.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The Src++ cell line was derived from a control mouse embryo. The cells were immortalized by infection with a retroviral vector transducing the SV40 large T antigen. xa0
Comments：	The Src++ cell line expresses endogenous wild type tyrosine kinase Src, but lacks expression of the tyrosine kinases Yes and Fyn.xa0 Cells deficient for Src, Yes, and Fyn are available as SYF (ATCC CRL-2459).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C
Name of Depositor：	RA Klinghoffer, P Soriano
Deposited As：	mouse
References：	Klinghoffer RA, et al. Src family kinases are required for integrin but not PDGFR signal transduction. EMBO J. 18: 2459-2471, 1999. PubMed: 10228160 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.