SL-29

规格:

货期:

编号:TS210881

品牌:Testobio

产品名称：	SL-29
商品货号：	TS210881
Organism：	Gallus gallus, chicken
Tissue：	embryo
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo; 11 days gestation
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	This cell line was developed by standard trypsinization of a decapitated 11-day SPAFAS leghorn embryo.
Comments：	The cells have a doubling potential of 35 population doublings.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: tryptose phosphate broth to a final concentration of 5% and fetal bovine serum to a final concentration of 5%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: An inoculation density of 5 X 10⁴ viable cells per cm² of flask or dish surface area is recommended Medium Renewal: 2 to 3 times per week Note: The tryptose phosphate broth should be prepared fresh every 2 weeks. Fresh glutamine is also very important for the growth of this line.
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Population Doubling Capacity：	The cells have a doubling potential of 35 population doublings.
Name of Depositor：	RJ Hay
Deposited As：	Gallus gallus
References：	. Aging in cell and tissue cultures. New York: Plenum Press; 1970. Ramirez AD, et al. Antisporozoite antibodies with protective and nonprotective activities: in vitro and in vivo correlations using Plasmodium gallinaceum, an avian model. J. Eukaryot. Microbiol. 42: 705-708, 1995. PubMed: 8520586

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SL-29

货号: TS210881
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产品名称：	SL-29
商品货号：	TS210881
Organism：	Gallus gallus, chicken
Tissue：	embryo
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo; 11 days gestation
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	This cell line was developed by standard trypsinization of a decapitated 11-day SPAFAS leghorn embryo.
Comments：	The cells have a doubling potential of 35 population doublings.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: tryptose phosphate broth to a final concentration of 5% and fetal bovine serum to a final concentration of 5%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: An inoculation density of 5 X 10⁴ viable cells per cm² of flask or dish surface area is recommended Medium Renewal: 2 to 3 times per week Note: The tryptose phosphate broth should be prepared fresh every 2 weeks. Fresh glutamine is also very important for the growth of this line.
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Population Doubling Capacity：	The cells have a doubling potential of 35 population doublings.
Name of Depositor：	RJ Hay
Deposited As：	Gallus gallus
References：	. Aging in cell and tissue cultures. New York: Plenum Press; 1970. Ramirez AD, et al. Antisporozoite antibodies with protective and nonprotective activities: in vitro and in vivo correlations using Plasmodium gallinaceum, an avian model. J. Eukaryot. Microbiol. 42: 705-708, 1995. PubMed: 8520586