| 产品名称： | SNU-423 |
|---|---|
| 商品货号： | TS210887 |
| Organism： | Homo sapiens, human |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain Hepatitis B virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade III/IV, pleomorphic hepatocellular carcinoma |
| Age： | 40 years |
| Gender： | male |
| Ethnicity： | Asian |
| Karyotype： | aneuploid; modal number = 79 |
| Derivation： | SNU-423 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus doxorubicin.
Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. |
| Clinical Data： | 40 years
Asian
male
|
| Antigen Expression： | Blood Type B; Rh + |
| Comments： | Grossly, the original tumor was single nodular with perinodular extensions.
Histologically, it was trabecular type.
The cultured cells are multinucleated. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.
HBV genomic RNA was not expressed. |
| Complete Growth Medium： | RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 10,13 D16S539: 9 D5S818: 10 D7S820: 12 THO1: 6,9 TPOX: 8 vWA: 15 |
| Population Doubling Time： | 72 hrs |
| Name of Depositor： | J Park |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1990 |
| References： | Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080 |
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| 产品名称： | SNU-423 |
|---|---|
| 商品货号： | TS210887 |
| Organism： | Homo sapiens, human |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain Hepatitis B virus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade III/IV, pleomorphic hepatocellular carcinoma |
| Age： | 40 years |
| Gender： | male |
| Ethnicity： | Asian |
| Karyotype： | aneuploid; modal number = 79 |
| Derivation： | SNU-423 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus doxorubicin.
Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum. |
| Clinical Data： | 40 years
Asian
male
|
| Antigen Expression： | Blood Type B; Rh + |
| Comments： | Grossly, the original tumor was single nodular with perinodular extensions.
Histologically, it was trabecular type.
The cultured cells are multinucleated. Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.
HBV genomic RNA was not expressed. |
| Complete Growth Medium： | RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 10,13 D16S539: 9 D5S818: 10 D7S820: 12 THO1: 6,9 TPOX: 8 vWA: 15 |
| Population Doubling Time： | 72 hrs |
| Name of Depositor： | J Park |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1990 |
| References： | Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080 |
